RECEPTOR-MEDIATED ENTRY OF PSEUDOMONAS TOXIN - METHYLAMINE BLOCKS CLUSTERING STEP
RECEPTOR-MEDIATED ENTRY OF PSEUDOMONAS TOXIN - METHYLAMINE BLOCKS CLUSTERING STEP
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DOI：
10.1128/iai.40.2.806-811.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
SAELINGER, CB
中科院分区：
文献类型：
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作者：
MORRIS, RE;MANHART, MD;SAELINGER, CB
使用生物素-PE 和亲和素-金观察假单胞菌外毒素 A (PE) 与小鼠 LM 成纤维细胞的关联。 PE 进入涂层区域的运动发生在单层升温至 37°C 的 30 秒内。 C. 这种聚集被伯胺 CH3NH2 和 NH4Cl 终止，但没有被叔胺氯喹改变。毒素内化速度很快，半衰期为.apprx。 5分钟尽管伯胺停止聚集，但它们并没有改变毒素内化的速率；他们确实改变了进入后的路线。甲胺可能通过阻止聚集来保护细胞免受 PE 的致命作用，至少部分如此。 PE毒性的有效表达可能需要受体介导的内吞作用。
The association of Pseudomonas exotoxin A (PE) with mouse LM fibroblasts was visualized by using biotinyl-PE and avidin-gold. Movement of PE into coated regions occurred within 30 s of warming monolayers to 37.degree. C. This clustering was stopped by the primary amines CH3NH2 and NH4Cl but was not altered by the tertiary amine chloroquine. Toxin internalization was rapid, with a half-time of .apprx. 5 min. Although primary amines stopped clustering, they did not alter the rate of toxin internalization; they did alter the route followed after entry. Methylamine possibly protects cells from the lethal action of PE, at least in part, by blocking clustering; receptor-mediated endocytosis may be required for efficient expression of PE toxicity.