Mammalian chymotrypsin-like enzymes. Comparative reactivities of rat mast cell proteases, human and dog skin chymases, and human cathepsin G with peptide 4-nitroanilide substrates and with peptide chloromethyl ketone and sulfonyl fluoride inhibitors.
Mammalian chymotrypsin-like enzymes. Comparative reactivities of rat mast cell proteases, human and dog skin chymases, and human cathepsin G with peptide 4-nitroanilide substrates and with peptide chloromethyl ketone and sulfonyl fluoride inhibitors.
复制标题
哺乳动物胰凝乳蛋白酶样酶。
DOI：
10.1021/bi00329a037
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Lazarus,GG
中科院分区：
文献类型：
--
作者：
Powers,JC;Tanaka,T;Harper,JW;Minematsu,Y;Barker,L;Lincoln,D;Crumley,KV;Fraki,JE;Schechter,NM;Lazarus,GG
材料和方法 RMCP I 和 II 的制备如前所述（Everitt & Neurath，1979；Woodbury & Neurath，1978；Woodbury 等人，1981）。通过鱼精蛋白沉淀和凝胶过滤色谱从皮肤的高盐提取物中纯化人和狗的皮肤食糜酶（Schechter 等，1983）。在这个纯化阶段，它们似乎没有被其他蛋白酶污染。人类白细胞组织蛋白酶 G 是 Dr. 的礼物。 J. Travis 和他在佐治亚大学的研究小组。牛胰凝乳蛋白酶 Aa、TV-苯甲酰基-L-酪氨酸乙酯和凝乳抑素购自 Sigma Chemical Co., St. Louis, MO。这
Materials and Methods RMCP I and II were prepared as previously described (Everitt & Neurath, 1979; Woodbury & Neurath, 1978; Woodbury et al., 1981). Human and dog skin chymases were purified from the high-salt extract of skin by protamine pre-cipitation followed by gel filtration chromatography (Schechter et al., 1983). At this stage of purification, theydo not appear to be contaminated by other proteases. Human leukocyte cathepsin G was a gift fromDr. J. Travis and his research group at the University of Georgia. Bovine chymotrypsin Aa, TV-benzoyl-L-tyrosine ethyl ester, and chymostatin were pur-chased from Sigma Chemical Co., St. Louis, MO. The