Insulin stimulates androgen accumulation in incubations of human ovarian stroma and theca.
Insulin stimulates androgen accumulation in incubations of human ovarian stroma and theca.
复制标题
胰岛素刺激人卵巢基质和卵泡膜孵化过程中雄激素的积累。
DOI：
10.1097/00006250-198409001-00019
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发表时间：
1984
影响因子：
7.2
通讯作者：
Ryan,KJ
中科院分区：
文献类型：
--
作者：
Barbieri,RL;Makris,A;Ryan,KJ
胰岛素对卵巢类固醇合成的影响在4天的培养中进行了研究，培养的基质和卵泡膜来自一名患有高雄激素血症、胰岛素抵抗和黑棘皮病的女性，以及一名正常骑自行车的女性。从高雄激素血症、胰岛素抵抗和黑棘皮病患者中获得的卵泡膜孵育中，单独使用促黄体生成素（LH）（25 ng/mL）刺激雄烯二酮、睾酮、孕酮和雌二醇蓄积。胰岛素（500 ng/mL）单独刺激雄烯二酮和睾酮的积累，但不是孕酮或雌二醇的积累。在从高雄激素血症、胰岛素抵抗和黑棘皮病患者中获得的基质的孵育中，单独LH（25 ng/mL）刺激雄烯二酮和睾酮积累，但不刺激二氢睾酮积累。胰岛素（500 ng/mL）单独刺激雄烯二酮、睾酮和双氢睾酮蓄积。在正常的周期性妇女的间质孵育，LH加胰岛素协同作用，刺激雄烯二酮的积累。这些结果表明，胰岛素可能是一个调节类固醇生物合成的卵泡膜和基质室的人卵巢。（妇产科64：73 S，1984）
: The effects of insulin on ovarian steroidogenesis were examined in four-day incubations of minced stroma and theca obtained from a woman with hyperandrogenism, insulin resistance, and acanthosis nigricans, and from a normally cycling woman. In incubations of theca obtained from the patient with hyperandrogenism, insulin resistance, and acanthosis nigricans, lutenizing hormone (LH)(25 ng/mL) alone stimulated androstenedione, testosterone, progesterone, and estradiol accumulation. Insulin (500 ng/mL) alone stimulated androstenedione and testosterone accumulation, but not progesterone or estradiol accumulation. In incubations of stroma obtained from the hyperandrogenism, insulin resistance, and acanthosis nigricans patient, LH (25 ng/mL) alone stimulated androstenedione and testosterone accumulation, but not dihydrotestosterone accumulation. Insulin (500 ng/mL) alone stimulated androstenedione, testosterone, and dihydrotestosterone accumulation. In incubations of stroma from the normally cycling woman, LH plus insulin acted synergistically to stimulate androstenedione accumulation. These results suggest that insulin may be a regulator of steroid biosynthesis in the thecal and stromal compartments of the human ovary.(Obstet Gynecol 64: 73S, 1984)