Design and bioinformatics analysis of novel biomimetic peptides as nanocarriers for gene transfer
Design and bioinformatics analysis of novel biomimetic peptides as nanocarriers for gene transfer
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DOI：
10.7508/nmj.2015.01.003
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发表时间：
2015-12-01
影响因子：
1.5
通讯作者：
Hosseinkhani, Saman
中科院分区：
文献类型：
--
作者：
Majidi, Asia;Nikkhah, Maryam;Hosseinkhani, Saman
目的(S)：核酸分子的大小和电荷严重阻碍了将核酸引入细胞用于治疗目的，因此需要有效的载体来帮助细胞摄取。多年来，人们一直致力于基于生物结构域的重组载体的研究，这些重组载体在基因治疗中具有潜在的应用前景。这些载体是通过基因工程的方法合成的，从而产生了具有自然界中不存在的新性质的生物大分子。材料和方法：在本研究中，我们利用同源建模设计了新的多肽，目的是克服细胞屏障，通过生物信息学工具成功地传递基因。结果：结果表明，该载体能有效地将pDNA凝聚成平均粒径约100 nm的纳米颗粒。结论：我们希望这些多肽能够克服基因转移的生物屏障，并介导高效的基因传递。
Objective(s): The introduction of nucleic acids into cells for therapeutic objectives is significantly hindered by the size and charge of these molecules and therefore requires efficient vectors that assist cellular uptake. For several years great efforts have been devoted to the study of development of recombinant vectors based on biological domains with potential applications in gene therapy. Such vectors have been synthesized in genetically engineered approach, resulting in biomacromolecules with new properties that are not present in nature.Materials and Methods: In this study, we have designed new peptides using homology modeling with the purpose of overcoming the cell barriers for successful gene delivery through Bioinformatics tools. Three different carriers were designed and one of those with better score through Bioinformatics tools was cloned, expressed and its affinity for pDNA was monitored.Results: The resultszz demonstrated that the vector can effectively condense pDNAinto nanoparticles with the average sizes about 100 nm.Conclusion: We hope these peptides can overcome the biological barriers associated with gene transfer, and mediate efficient gene delivery.