Effects of Cinnamoyloxy-mammeisin from Geopropolis on Osteoclast Differentiation and Porphyromonas gingivalis-Induced Periodontitis.
Effects of Cinnamoyloxy-mammeisin from Geopropolis on Osteoclast Differentiation and Porphyromonas gingivalis-Induced Periodontitis.
复制标题
Geopropolis 中的肉桂酰氧基玛迈辛对破骨细胞分化和牙龈卟啉单胞菌诱发的牙周炎的影响。
DOI：
10.1021/acs.jnatprod.7b00194
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发表时间：
2017
影响因子：
5.1
通讯作者：
Rosalen,PedroLuiz
中科院分区：
文献类型：
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作者：
daCunha,MarcosGuilherme;Ramos-Junior,ErivanSchnaider;Franchin,Marcelo;Taira,ThaiseMayumi;Beutler,JohnA;Franco,GilsonCesarNobre;Ikegaki,Masaharu;deAlencar,SeverinoMatias;Fukada,SandraYasuyo;Rosalen,PedroLuiz
Bone-loss-related diseases such as rheumatoid arthritis, osteomyelitis, osteoporosis, and periodontitis are associated with high rates of morbidity worldwide. These disorders are characterized by an imbalance between the formation and activity of osteoblasts and osteoclasts, leading to bone loss. In this context, we evaluated the effect of cinnamoyloxy-mammeisin (CNM), an anti-inflammatory coumarin found inMelipona scutellarisgeopropolis, on key targets related to bone remodeling. In the present study we investigated thein vitroeffects of CNM on osteoclast differentiation and M-CSF+RANKL-induced osteoclastogenic marker expression. Additionally, the interference of CNM treatment on osteoclast activity was evaluated by zymography and resorption area. Finally, we assessed the capacity of the compound to mitigate alveolar bone lossin vivoin experimental murine periodontitis induced byPorphyromonas gingivalis. We observed that treatment with CNM impaired osteoclast differentiation, as evidenced by a reduced number of tartrate-resistant acid-phosphatase-positive multinucleated cells (TRAP+) as well as the expression of osteoclastogenic markers upon M-CSF+RANKL-induced stimulation. Similarly, we observed reduced gelatinolytic and resorption capacity in M-CSF+RANKL-induced cellsin vitro. Lastly, CNM attenuated alveolar bone loss in an experimental murine periodontitis model. These findings indicate that CNM may be considered a promising treatment for bone loss diseases.
