Quantification of IncP-1 Plasmid Prevalence in Environmental Samples
Quantification of IncP-1 Plasmid Prevalence in Environmental Samples
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DOI：
10.1128/aem.03728-12
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发表时间：
2013-02-01
影响因子：
4.4
通讯作者：
Heuer, Holger
中科院分区：
文献类型：
--
作者：
Jechalke, Sven;Dealtry, Simone;Heuer, Holger
为了研究广泛宿主范围的IncP-1质粒在细菌对不规则环境挑战的适应性中的作用，开发了一种定量实时PCR测定，其特异性地检测环境样品中所有IncP-1质粒中保守的科尔布基因。分析了IncP-1质粒在农药废水生物净化系统中的动态变化。
To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.