In vivo imaging and biochemical characterization of protease function using fluorescent activity-based probes.
In vivo imaging and biochemical characterization of protease function using fluorescent activity-based probes.
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DOI：
10.1002/9780470559277.ch120235
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Bogyo, Matthew
中科院分区：
文献类型：
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作者：
Edgington, Laura E;Bogyo, Matthew
Activity-based probes (ABPs) are reactive small molecules that covalently bind to active enzymes. When tagged with a fluorophore, ABPs serve as powerful tools to investigate enzymatic activity across a wide variety of applications. In this article, detailed protocols are provided for using fluorescent ABPs to biochemically characterize the activity of proteases in vitro. Furthermore, descriptions are provided of how these probes can be applied to image protease activity in live animals and tissues along with subsequent analysis by histology, flow cytometry, and SDS-PAGE.
