Nicotinamide mononucleotide adenylyltransferase maintains active zone structure by stabilizing Bruchpilot.
Nicotinamide mononucleotide adenylyltransferase maintains active zone structure by stabilizing Bruchpilot.
复制标题
烟酰胺单核苷酸腺苷酸转移酶通过稳定 Bruchpilot 来维持活性区结构。
DOI：
10.1038/embor.2012.181
复制
发表时间：
2013
期刊：
影响因子：
7.7
通讯作者：
Zhai,RGrace
中科院分区：
文献类型：
--
作者：
Zang,Shaoyun;Ali,YousufO;Ruan,Kai;Zhai,RGrace
Active zones are specialized presynaptic structures critical for neurotransmission. We show that a neuronal maintenance factor, nicotinamide mononucleotide adenylyltransferase (NMNAT), is required for maintaining active zone structural integrity inDrosophilaby interacting with the active zone protein, Bruchpilot (BRP), and shielding it from activity‐induced ubiquitin–proteasome‐mediated degradation. NMNAT localizes to the peri‐active zone and interacts biochemically with BRP in an activity‐dependent manner. Loss of NMNAT results in ubiquitination, mislocalization and aggregation of BRP, and subsequent active zone degeneration. We propose that, as a neuronal maintenance factor, NMNAT specifically maintains active zone structure by direct protein–protein interaction.
