Construction of sandwiched self-powered biosensor based on smart nanostructure and capacitor: Toward multiple signal amplification for thrombin detection
Construction of sandwiched self-powered biosensor based on smart nanostructure and capacitor: Toward multiple signal amplification for thrombin detection
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DOI：
10.1016/j.snb.2019.127418
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发表时间：
2020-02
影响因子：
8.4
通讯作者：
Yihan Wang;Fu-ting Wang;Zi-wei Han;Ke-Jing Huang;Xuemei Wang;Zhen-hua Liu;Shu-yu Wang;Yun-fei Lu
中科院分区：
文献类型：
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作者：
Yihan Wang;Fu-ting Wang;Zi-wei Han;Ke-Jing Huang;Xuemei Wang;Zhen-hua Liu;Shu-yu Wang;Yun-fei Lu
基于电容信号放大和单室葡萄糖/O2酶生物燃料电池（EBFC），构建了一种自供电的蛋白质电化学传感平台。制备了SnS 2纳米花/Au纳米颗粒（AuNPs）和DNA-碳纳米管生物缀合物/AuNPs，并将其修饰在碳纸上作为电极基底。精心设计了用于制作生物阳极的夹层结构，以显著提高装置的开路电压，该开路电压由与含有葡萄糖氧化酶和适体的DNA生物缀合物偶联的蛋白质触发。基于EBFC的适体传感器随后将在靶凝血酶（TB）的存在下催化葡萄糖氧化。更重要的是，商业电容器也被引入EBFC中，以积累更高瞬时电流的电荷。这将导致显著放大的读出信号，其可以被数字放大器有效地捕获。开路电压可以调节，目标TB浓度范围为0.02至5 ng/mL，检测限为7.90 pg/mL。EBFC对电容器充电后，放电灵敏度可达42.4 μA/（ng/mL），比纯EBFC提高了18.4倍。此外，这种电容器/EBFC混合装置可以扩展为其他蛋白质定量测定的通用平台，具有很大的临床分析潜力。
A self-powered electrochemical sensing platform is fabricated to sensitively detect protein based on capacitor signal amplification and one-compartment glucose/O2enzymatic biofuel cells (EBFCs). SnS2nanoflowers/Au nanoparticles (AuNPs) and DNA-carbon nanotubes bioconjugate/AuNPs are prepared and modified on carbon paper as electrode substrates. The sandwiched structure to craft bioanodes is elaborately designed to dramatically enhance the open circuit voltage of the device, triggered by protein coupled with the DNA bioconjugate containing glucose oxidase and aptamer. The EBFCs-based aptasensor will subsequently catalyze glucose oxidation in the presence of the target thrombin (TB). More importantly, a commercial capacitor is also introduced into EBFCs to accumulate charge for a higher instantaneous current. This will result in a significantly amplified readout signal, which can be efficiently captured by digital multimeter. The open circuit voltage can be tuned with target TB concentration ranging from 0.02 to 5 ng/mL with a low detection limit of 7.90 pg/mL. Once the capacitor is charged by EBFCs, a sensitivity of 42.4 μA/(ng/mL) can be discharged with an increase of 18.4 times than that of pure EBFCs. Furthermore, this capacitor/EBFC hybrid device can be extended as a common platform for the quantitative determination of other proteins and has a great clinical analysis potential.