Fluorescent labeled anti-EGFR antibody for identification of regional and distant metastasis in a preclinical xenograft model
Fluorescent labeled anti-EGFR antibody for identification of regional and distant metastasis in a preclinical xenograft model
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DOI：
10.1002/hed.20782
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发表时间：
2008-06-01
影响因子：
2.9
通讯作者：
Rosenthal, Eben L.
中科院分区：
文献类型：
--
作者：
Gleysteen, John P.;Newman, J. Robert;Rosenthal, Eben L.
背景资料。区域和远处转移性疾病的检测对患者管理具有重要意义。荧光成像可能是一种有用的转移检测和切除技术。用近红外荧光团(Cy5.5)标记抗表皮生长因子受体抗体(西妥昔单抗)和免疫球蛋白G(免疫球蛋白G)，然后系统地给经口内或静脉注射头颈部鳞状细胞癌所致肿瘤的小鼠体内注射。在接受荧光立体显微镜检查以评估肺或颈部淋巴结转移之前，处死小鼠。连续切除荧光区，直到创面显示阴性荧光。注射Ig G-Cy5.5后，肺转移瘤小鼠呈弥漫背景，注射西妥昔单抗-Cy5.5后，小鼠肺表面出现斑点状荧光。颈转移瘤小鼠的舌癌和双侧颈淋巴结均可见清晰的荧光。结论：荧光与组织病理学相关。这些数据表明，西妥昔单抗-Cy5.5可能在检测和指导切除局部和远处微转移方面有临床应用价值。(C)2008年威利期刊公司。
Background. Detection of regional and distant metastatic disease has significant implications for patient management. Fluorescent imaging may be a useful technique for metastasis detection and removal.Methods. Anti-epidermal growth factor receptor antibody (cetuximab) and isotype-matched control antibody (immunoglobulin G [IgG]) were labeled with a near-infrared fluorophore (Cy5.5), then systemically administered to mice with tumors resulting from either intraoral or intravenous injections of head and neck squamous cell carcinoma. Mice were sacrificed before undergoing fluorescent stereomicroscopy to assess pulmonary or cervical lymph node metastasis. Fluorescent areas were serially excised until wound bed demonstrated negative fluorescence.Results. Mice bearing pulmonary metastases displayed diffuse background after IgG-Cy5.5 injection, but demonstrated a speckled fluorescent pattern across lung surface following cetuximab-Cy5.5 injection. Mice bearing cervical metastases demonstrated clear fluorescence of primary tongue tumor and bilateral cervical nodes. Fluorescence correlated with histopathology.Conclusion. These data suggest that cetuximab-Cy5.5 may have clinical utility in the detection and guided the removal of regional and distant micrometastasis. (C) 2008 Wiley Periodicals, Inc.