Guanosine 3',5'-cyclic nucleotide binding proteins of bovine retina identified by photoaffinity labeling.
Guanosine 3',5'-cyclic nucleotide binding proteins of bovine retina identified by photoaffinity labeling.
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通过光亲和标记鉴定牛视网膜的鸟苷 3,5-环核苷酸结合蛋白。
DOI：
10.1073/pnas.87.6.2201
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发表时间：
1990
影响因子：
11.1
通讯作者：
Khorana,HG
中科院分区：
文献类型：
--
作者：
Thompson,DA;Khorana,HG
用8-叠氮基-[32P]cGMP进行光亲和标记，鉴定了牛视网膜膜部分，特别是视杆细胞外节(ROS)中存在的环GMP结合蛋白。两种可溶性蛋白和两种膜结合蛋白被特异性标记。可溶性蛋白分别为93和72 kDa，分别对应于ROS、cGMP磷酸二酯酶和cGMP依赖蛋白激酶的α亚基。两种膜相关蛋白中的一种，53 kDa，存在于所有视网膜颗粒组分中。它的功能尚不清楚。它不同于cAMP依赖的蛋白激酶，也不同于ROS的63 kDa cGMP激活通道。第二个膜相关蛋白，37 kDa，只存在于不含ROS的组分中。这种蛋白的分子质量类似于以前被认为是杆状细胞的cGMP结合蛋白。
Cyclic GMP-binding proteins present in membrane fractions of bovine retina and, in particular, rod outer segments (ROS) were identified by photoaffinity labeling with 8-azido-[32P]cGMP. Two soluble proteins and two membrane-associated proteins were specifically labeled. The soluble proteins, 93 and 72 kDa, corresponded respectively to the alpha subunit of ROS cGMP phosphodiesterase and cGMP-dependent protein kinase. One of the two membrane-associated proteins, 53 kDa, was present in all particulate retinal fractions. Its function is unknown. It is distinct from cAMP-dependent protein kinase or the 63-kDa cGMP-activated channel from ROS. The second membrane-associated protein, 37 kDa, was present only in fractions that did not contain ROS. The molecular mass of this protein was similar to that of a cGMP-binding protein previously attributed to rod cells.