Synchronized oscillation of the segmentation clock gene in vertebrate development
Synchronized oscillation of the segmentation clock gene in vertebrate development
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DOI：
10.1007/s00285-009-0296-1
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发表时间：
2010-08
影响因子：
1.9
通讯作者：
Koichiro Uriu;Y. Morishita;Y. Iwasa
中科院分区：
文献类型：
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作者：
Koichiro Uriu;Y. Morishita;Y. Iwasa
在脊椎动物的体节发生中，“分段时钟”基因（如斑马鱼的hera、小鼠的hesin和鸡的hairin）显示出振荡，通过细胞间的相互作用与附近的细胞同步。在斑马鱼中，相邻细胞通过Delta-Notch信号相互作用以实现同步。然而，在Delta-Notch下，具有高表达的分段时钟基因的细胞倾向于抑制其在相邻细胞中的表达，这可能产生空间异质性而不是同步振荡。在这里，我们研究了条件下，前体中胚层细胞显示同步振荡的基因表达数学。我们采用了一个模型，该模型明确考虑了分割时钟基因的mRNA和蛋白质的动力学以及通过Delta-Notch信号传导的细胞-细胞相互作用。从随机生成参数的模型的统计研究中，我们揭示了系统产生稳定同步振荡的可能性如何取决于基因-蛋白质动力学中每个反应的速率。
In vertebrate somitogenesis, “segmentation clock” genes (herin zebrafish,hesin mouse, andhairyin chick) show oscillation, synchronized over nearby cells through intercellular interaction. In zebrafish, neighboring cells interact by Delta-Notch signaling to realize synchronization. Under Delta-Notch, however, a cell with a high expression of the segmentation clock gene tends to suppress its expression in adjacent cells, which might produce spatial heterogeneity instead of synchronized oscillation. Here we studied the conditions under which pre-somitic mesoderm cells show synchronized oscillation of gene expression mathematically. We adopted a model that explicitly considers the kinetics of the mRNA and proteins of the segmentation clock gene and cell–cell interaction via Delta-Notch signaling. From statistical study of a model with randomly generated parameters, we revealed how the likelihood that the system generates stable synchronized oscillation depends on the rate of each reaction in the gene–protein kinetics.