Inhibitory effect of voglibose and gymnemic acid on maltose absorption in vivo.
Inhibitory effect of voglibose and gymnemic acid on maltose absorption in vivo.
复制标题
伏格列波糖和匙羹藤酸对体内麦芽糖吸收的抑制作用。
DOI：
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发表时间：
2001
影响因子：
4.3
通讯作者：
Yasutake Hiji
中科院分区：
文献类型：
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作者：
Hong Luo;Toshiaki Imoto;Yasutake Hiji
目的
为了确定伏格列波糖和匙羹藤酸（GA）的组合是否可以改善糖尿病护理，我们比较了伏格列波糖和GA对小肠中麦芽糖吸收的组合和单独作用。
方法
取Wistar大鼠Treitz韧带尾侧2cm处长30 cm的小肠作原位肠袢，用麦芽糖（10 mmol/L）加或不加不同剂量的伏格列波糖和/或GA，随机循环灌注1h。为了比较时间过程，重复灌注10 mmol/L麦芽糖四次。每次持续1小时，隔30分钟冲洗。除对照组外，第一次试验中，GA（0.5g/L）和伏格列波糖（2 μ mol/L）的浓度均较低。
结果
麦芽糖的吸收率以联合组最低（P<0.05，ANOVA），如0.5g/L-GA和2 μ mol/L伏格列波糖加10 mmol/L麦芽糖灌流后第1小时的抑制率约为37%。联合用药起效时间缩短至30 min，有效持续时间延长至4 h，因此有效持续时间内麦芽糖吸收总量的抑制作用比单独用药更显著（P < 0.05，Mann Whitney U检验）。GA对肠道吸收屏障的影响在其联合作用中起重要作用。
结论
伏格列波糖和GA的作用增强。糖尿病的管理可以通过使用该组合来改善。
AIM
To determine whether diabetic care can be improved by combination of voglibose and gymnemic acid (GA), we compared the combinative and individual effects of voglibose and GA on maltose absorption in small intestine.
METHODS
The small intestine 30 cm long from 2 cm caudal ward Treitz's ligament of Wistar rat was used as an in situ loop, which was randomly perfused in recircular mode with maltose (10mmol/L) with or without different dosages of voglibose and/or GA for an hour. To compare the time course, perfusion of 10 mmol/L maltose was repeated four times. Each time continued for 1 hour and separated by 30 minutes rinse. In the first time, lower dosages of GA (0.5g/L) and/or voglibose (2 micromol/L) were contained except control.
RESULTS
Absorptive rate of maltose was the lowest in combinative group (P<0.05, ANOVA), for example, the inhibition rate was about 37% during the first hour when 0.5 g/L-GA and 2 micromol/L voglibose with 10 mmol/L maltose were perfused in the loop. The onset time was shortened to 30 minutes and the effective duration was prolonged to 4 hours with the combination; therefore the total amount of maltose absorption during the effective duration was inhibited more significantly than that in the individual administration (P < 0.05, U test of Mann Whitney). The effect of GA on absorptive barriers of the intestine played an important role in the combinative effects.
CONCLUSION
There are augmented effects of voglibose and GA. The management of diabetes mellitus can be improved by employing the combination.