Advances in microbeam technologies and applications to radiation biology
Advances in microbeam technologies and applications to radiation biology
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DOI：
10.1093/rpd/ncv192
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发表时间：
2015-09-01
影响因子：
1
通讯作者：
Seznec, H.
中科院分区：
文献类型：
--
作者：
Barberet, P.;Seznec, H.
带电粒子微束(CPM)允许通过计数的高能离子来对准亚细胞隔间。虽然CPM最初是在20世纪90年代末开发的，目的是克服宽束辐射不可避免地导致每细胞穿越次数的统计波动，但CPM已经引起了越来越多的兴趣，现在已被广泛用于辐射生物学研究。除了多年来在这些设备的应用中盛行的低剂量细胞反应的研究外，最近还出现了几个新的课题。通过将它们在微米体积内产生高度聚集损伤的能力与免疫染色或活细胞GFP标记相结合，已经引入了监测辐射诱导的DNA损伤和修复的巨大潜力。这种类型的研究将终端站推向了先进的荧光显微镜技术，目前有几条微束线配备了最先进的延时荧光成像显微镜。此外，CPM现在也被用来以高度受控的方式照射多细胞模型。本文综述了带电粒子微束的最新发展及其在辐射生物学中的应用。
Charged-particle microbeams (CPMs) allow the targeting of sub-cellular compartments with a counted number of energetic ions. While initially developed in the late 1990s to overcome the statistical fluctuation on the number of traversals per cell inevitably associated with broad beam irradiations, CPMs have generated a growing interest and are now used in a wide range of radiation biology studies. Besides the study of the low-dose cellular response that has prevailed in the applications of these facilities for many years, several new topics have appeared recently. By combining their ability to generate highly clustered damages in a micrometric volume with immunostaining or live-cell GFP labelling, a huge potential for monitoring radiation-induced DNA damage and repair has been introduced. This type of studies has pushed end-stations towards advanced fluorescence microscopy techniques, and several microbeam lines are currently equipped with the state-of-the-art time-lapse fluorescence imaging microscopes. In addition, CPMs are nowadays also used to irradiate multicellular models in a highly controlled way. This review presents the latest developments and applications of charged-particle microbeams to radiation biology.