CHEMICAL MODIFICATION OF DEOXYRIBONUCLEIC ACIDS - A DIRECT STUDY BY C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY
CHEMICAL MODIFICATION OF DEOXYRIBONUCLEIC ACIDS - A DIRECT STUDY BY C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY
复制标题
DOI：
10.1021/jo00174a002
复制
发表时间：
1983-01-01
影响因子：
3.6
通讯作者：
BYRN, SR
中科院分区：
文献类型：
--
作者：
CHANG, CJ;GOMES, JD;BYRN, SR
Please try later.
The chemical modifications of salmon sperm and salmon testes DNA with 90% 13C-enriched methyl methanesulfonate were directly studied by 13C NMR and 31P NMR. This direct stable isotope approach eliminates all tedious degradation and separation processes for determining the reactive sites and product distribution in studying the in vitro interaction between biological macromolecules and bioactive compounds. Seven methylated products, 7-methyldeoxyguanosine, 1-methyldeoxyadenosine, 3-methyldeoxycytidine, 1-methyldeoxyguanosine, 3-methylthymidine, methyl phosphodiester and methyl phosphotriester were determined by comparison with model compounds and extensive study of the chemical properties of the methylated DNA. The relative specificity of the methylation reactions may correlate with the DNA conformation.