Reactive astrocytes increase expression of proNGF in the mouse model of contused spinal cord injury
Reactive astrocytes increase expression of proNGF in the mouse model of contused spinal cord injury
复制标题
反应性星形胶质细胞增加小鼠脊髓挫伤模型中 proNGF 的表达
DOI：
10.1016/j.neures.2019.07.007
复制
发表时间：
2020-08-01
影响因子：
2.9
通讯作者：
Chen, Bei-Yu
中科院分区：
文献类型：
--
作者：
Cheng, Ying-Ying;Zhao, Hai-Kang;Chen, Bei-Yu
Astrocytes are major glial cells critically in maintaining stability of the central nervous system and functional activation of astrocytes occurs rapidly in various diseased or traumatic events. We are interested in functional changes of astrocytes during the spinal cord injury, and studied expression of nerve growth factor (NGF) in activated astrocytes by mouse model of contused spinal cord injury and cell culture experiment. It revealed that the spinal cord injury resulted in apparent activation of astrocytes and microglial cells and decreased BMS scores. A larger number of astrocytes showed immunoreactivity to proNGF in the injured spinal cord areas, and proNGF expression increased and remained high level at 7 to 14dpi, which was coincided with upregulation of glial fibrillary acidic protein. The proNGF was clearly localized in both exosome-like vesicles and cytoplasm of astrocytes in culture. Electron microscopy confirmed exosome-like vesicles with proNGF-immunoreactivity in diameter sizes of 50-100 nm. Finally, cell culture with lipopolysaccharide (LPS) experiment indicated increasing expression and release of proNGF in the astrocytes with LPS exposure. This study demonstrated that reactive astrocytes increased proNGF expression after spinal cord injury, also suggesting involvement of exosome-like proNGF transport or release in triggering neuronal apoptosis and aggravating progression of spinal cord injury. (C) 2019 The Authors. Published by Elsevier B.V.
