Cytokine patterns in nasal secretion of non-atopic patients distinguish between chronic rhinosinusitis with or without nasal polys.
Cytokine patterns in nasal secretion of non-atopic patients distinguish between chronic rhinosinusitis with or without nasal polys.
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DOI：
10.1186/s13223-016-0123-3
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Gröger M
中科院分区：
文献类型：
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作者：
König K;Klemens C;Haack M;Nicoló MS;Becker S;Kramer MF;Gröger M
慢性鼻窦炎（CRS）是最常见的鼻部疾病之一，可分为CRS伴鼻息肉（NP）和CRS不伴鼻息肉（CRSsNP）。CRSsNP本身表现为TH 1环境和中性粒细胞浸润，而NP的特征在于混合的TH 1/TH 2特征和主要是嗜酸性粒细胞、浆细胞和肥大细胞的流入。为了发现疾病特异性细胞因子谱的目的，本研究比较CRSsNP、NP和健康对照之间鼻分泌物中介质和细胞因子的水平。该研究包括45名患有NP的参与者，48名患有CRSsNP的参与者和48名健康对照。过敏性鼻炎构成排除标准。使用棉絮法采集鼻分泌物样本，分别使用Bio-ELISA细胞因子试验或ELISA分析IL-4、IL-5、IL-10、IL-12、IL-13、IL-17、IL-8、GM-CSF、G-CSF、IFN-γ、MCP-1、MIP-1α、MIP-1β、嗜酸性粒细胞趋化因子和RANTES以及ECP和类胰蛋白酶。NP中检测到IL-5、IL-17、G-CSF、MCP-1、MIP-1α、MIP-1β、ECP和类胰蛋白酶水平升高，以及IL-10、IL-12、IL-13和IFN-γ水平降低。CRSsNP组RANTES和MIP-1β水平升高，IL-13水平降低。IL-4、IL-8、GM-CSF和嗜酸性粒细胞趋化因子在三组之间无差异。目前的工作表明，不平衡的TH 1和TH 2，连同下调调节性T淋巴细胞和上调TH 17在NP。此外，不同介质水平的升高代表了该疾病实体中各种炎性细胞的活化。然而，CRSsNP中的炎症在鼻分泌物中仅被微弱地描述。因此，鼻分泌物中的细胞因子可为鉴别诊断提供有用的信息。
Being one of the most common nasal diseases, chronic rhinosinusitis (CRS) is subdivided into CRS with nasal polyps (NP) and CRS without nasal polyps (CRSsNP). CRSsNP presents itself with a TH1 milieu and neutrophil infiltration, while NP is characterised by a mixed TH1/TH2 profile and an influx of predominantly eosinophils, plasma cells and mast cells. For the purpose of discovering disease-specific cytokine profiles, the present study compares levels of mediators and cytokines in nasal secretions between CRSsNP, NP, and healthy controls. The study included 45 participants suffering from NP, 48 suffering from CRSsNP and 48 healthy controls. Allergic rhinitis constituted an exclusion criterion. Nasal secretions, sampled using the cotton wool method, were analysed for IL-4, IL-5, IL-10, IL-12, IL-13, IL-17, IL-8, GM-CSF, G-CSF, IFN-γ, MCP-1, MIP-1α, MIP-1β, eotaxin, and RANTES, and for ECP and tryptase, using Bio-Plex Cytokine assay or ELISA, respectively. Elevated levels of IL-5, IL-17, G-CSF, MCP-1, MIP-1α, MIP-1β, ECP, and tryptase, as well as decreased levels of IL-10, IL-12, IL-13, and IFN-γ were detected in NP. CRSsNP presented increased levels of RANTES and MIP-1β while IL-13 was decreased. No differences between the three groups were found for IL-4, IL-8, GM-CSF, and eotaxin. The present work suggests a disequilibrium of TH1 and TH2, together with a down-regulation of regulatory T lymphocytes and up-regulated TH17 in NP. Moreover, elevated levels of diverse mediators represent the activation of various inflammatory cells in this disease entity. The inflammation in CRSsNP, however, is only weakly depicted in nasal secretions. Therefore, cytokines in nasal secretions may provide helpful information for differential diagnosis.