Phagocytic ability declines with age in adult Drosophila hemocytes.
Phagocytic ability declines with age in adult Drosophila hemocytes.
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DOI：
10.1111/acel.12227
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发表时间：
2014-08
期刊：
影响因子：
7.8
通讯作者：
Starz-Gaiano M
中科院分区：
文献类型：
--
作者：
Horn L;Leips J;Starz-Gaiano M
大多数多细胞生物表现出免疫功能随年龄增长的生理衰退。然而，人们对这些变化背后的机制知之甚少。我们研究了黑腹果蝇（Drosophila melanogaster）这一鉴定先天免疫和衰老影响基因的重要模型，以探讨吞噬作用在年龄相关免疫功能障碍中的作用。我们描述了1周龄和5周龄果蝇背血管免疫细胞对细菌感染的局部反应。我们开发了一种成年果蝇的定量吞噬实验，并利用它来表征年龄对转基因和自然变异系吞噬能力的影响。我们发现，在其他情况下，细菌吞噬所需的基因在成年果蝇中也是必需的。我们发现，来自年轻和年老果蝇的血细胞最初对细菌的吞噬能力相同，而来自年老果蝇的细胞会积累吞噬囊泡，因此破坏病原体的能力较差。我们的结果对于理解细胞过程的破坏如何随着年龄的增长影响免疫功能具有广泛的意义。
Most multicellular organisms show a physiological decline in immune function with age. However, little is known about the mechanisms underlying these changes. We examined Drosophila melanogaster, an important model for identifying genes affecting innate immunity and senescence, to explore the role of phagocytosis in age-related immune dysfunction. We characterized the localized response of immune cells at the dorsal vessel to bacterial infection in 1-week- and 5-week-old flies. We developed a quantitative phagocytosis assay for adult Drosophila and utilized this to characterize the effect of age on phagocytosis in transgenic and natural variant lines. We showed that genes necessary for bacterial engulfment in other contexts are also required in adult flies. We found that blood cells from young and old flies initially engulf bacteria equally well, while cells from older flies accumulate phagocytic vesicles and thus are less capable of destroying pathogens. Our results have broad implications for understanding how the breakdown in cellular processes influences immune function with age.