Cerebrospinal fluid levels of IgG, IgA and IgM in neurologic diseases
Cerebrospinal fluid levels of IgG, IgA and IgM in neurologic diseases
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神经系统疾病中脑脊液中 IgG、IgA 和 IgM 的水平
DOI：
10.1212/wnl.23.9.953
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发表时间：
1973
期刊：
影响因子：
9.9
通讯作者：
Geraldine Heintges
中科院分区：
文献类型：
--
作者：
J. Savory;Geraldine Heintges
脑脊液（CSF）组分的改变和疾病状态之间的关系一直是许多研究的主题。许多研究都涉及到蛋白质的分离，但不幸的是，这种技术缺乏灵敏度，不能测量单个蛋白质。更有用的电泳图谱显示γ球蛋白（IG）的相对比例升高，通常见于多发性硬化、亚急性硬化性全脑炎（SSPE）和神经系统炎性疾病。Link和Mullere最近利用放射免疫扩散这种更特异的技术报道了多发性硬化和神经系统感染病例中IgG、伊加和IgM的水平。其他工作者也使用放射免疫扩散法测定尿中的CSF中的IgA，但该技术缺乏精确度，并且在没有预先浓缩样品的情况下不具有足够的灵敏度来检测正常CSF中的伊加。电免疫扩散提供了优于前述方法的优点，因为分析可以直接在未浓缩的脊髓液上进行。Schuller最近的一份报告描述了一种电免疫扩散技术，其中伊加和IgM的灵敏度极限分别扩展到0.10和0.13 mg/100 ml。最近Laffin使用了一种相对简单的方法，他使用在窄口径玻璃管中的简单免疫扩散技术直接在未浓缩的C S F上测量IgG，如Oudin最初描述的：在1946年和最近由Oudin和Preer.Iy提出。这种非常灵敏的方法，每次测定只需要50 μ l样品，在我们的实验室2”中，已适用于测量未浓缩CSF中的IgG、伊加和IgM。已经发现该过程与涉及抗原-抗体复合物的光散射的免疫化学技术密切相关。本研究旨在评价Oudin免疫扩散法测定脑脊液中IgG、伊加和IgM的临床应用价值。
The relationship between alterations of cerebrospinal fluid (CSF) fractions and disease states has been the subject of numerous investigations. Many studies have involved separation of proteins by electrophoresis'-5 but unfortunately this technic lacks sensitivity and does not measure individual proteins. The more useful electrophoretic patterns show an elevation in the relative proportion of gamma globulin (Ig), typically seen in multiple sclerosis, subacute sclerosing panencephalitis (SSPE) and inflammatory diseases of the nervous system. Using the more specific technic of radial immunodiffusion, Link and Mullere recently reported levels o f IgG, IgA and IgM in cases of multiple sclerosis and infections of the nervous system. Other workers',X also have used radial immunodiffusion for measuring C S F i in in u ti ogl o bu I ins but this tech n ic I ac k s precision and does not possess sufficient sensitivity without prior concentration of the sample to detect IgA in normal CSF. Electroimmunodiffusion"l 5 offers an advantage over the previously mentioned methods i n that the analysis can be carried out directly on unconcentrated spinal fluid. A recent report by Schuller'" describes an electroimmunodiffusion technic where the limits of sensitivity of IgA and IgM have been extended t o 0.10 and 0.13 mg per 100 ml, respectively. A relatively simple procedure was used recently by Laffin'" who measured IgG directly on unconcentrated C S F using the technic of simple immunodiffusion in narrowbore glass tubes as described originally by Oudin': in 1946 and more recently by Oudin" and Preer.Iy This very sensitive procedure, which requires as little as 50 pl of specimen for each determination, has been adapted in our laboratory2" to the measurement of IgG, IgA and IgM in unconcentrated CSF. The procedure has been found to correlate closely with immunochemical technics involving light scattering of antigen-antibody complexes.""" The present study was undertaken to evaluate the clinical usefulness of the immunodiffusion technic of Oudin for studying levels of IgG, IgA and IgM in CSF.