ANTIOXIDANT DEFENSE MECHANISM IN PIGEON PEA UNDER COBALT STRESS

ANTIOXIDANT DEFENSE MECHANISM IN PIGEON PEA UNDER COBALT STRESS
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DOI:
10.1080/01904167.2013.849734
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发表时间:
2014-01
影响因子:
2.1
通讯作者:
R. Gopal
R. Gopal
中科院分区:
农林科学4区
文献类型:
--
作者:
R. Gopal

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研究了过量钴(Co;10~400μM Co)对木豆(Cajanus Cajan Mill)生长、生物量、钴积累、光合色素、脂质过氧化、脯氨酸、非蛋白硫醇和半胱氨酸含量以及抗氧化酶活性的影响。木豆叶片中叶绿素和类胡萝卜素浓度的降低与过氧化氢酶和超氧化物歧化酶活性的降低有关,表明过量钴具有抗过氧化作用。而抗坏血酸过氧化物酶和过氧化物酶活性的显著增加以及半胱氨酸、非蛋白硫醇和脯氨酸水平的增加则暗示了过量钴对抗氧化剂的诱导作用。木豆叶片中钴的毒性阈值(生长抑制10%)和毒性(生长抑制33%)值分别为叶中75和160μg−1,茎中42和180μg−1,根中50和340μg−1Co。
Influence of excess cobalt (Co; 10 to 400 μM Co) on growth, biomass, Co accumulation, photosynthetic pigments, lipid peroxidation, proline, non-protein thiols and cysteine contents as well as activities of anti-oxidative enzymes was studied in pigeon pea (Cajanus cajan Mill). In pigeon pea leaves decreased concentrations of chlorophyll and carotenoids on exposure to excess Co was associated with decrease activity of catalase and super oxide dismutase and suggest antiperoxidative nature of excess Co. However, a marked increase in the activities of ascorbate peroxidase and peroxidase and enhanced levels of cysteine, non-protein thiols, and proline are suggestive of induction of antioxidants in excess Co. The threshold of toxicity (10% growth reduction) and toxicity (33% growth reduction) values of Co in pigeon pea were 75 and 160 μg g−1in leaves, 42 and 180 μg g−1in stem and 50 and 340 μg g−1Co in roots, respectively.