High magnesium inhibits human osteoblast differentiation in vitro
High magnesium inhibits human osteoblast differentiation in vitro
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DOI：
10.1684/mrh.2011.0271
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发表时间：
2011-03-01
影响因子：
3.2
通讯作者：
Maier, Jeanette A. M.
中科院分区：
文献类型：
--
作者：
Leidi, Marzia;Dellera, Federica;Maier, Jeanette A. M.
在人类中的几项研究表明，高浓度和低浓度的镁对骨代谢和体内平衡都有有害影响。然而，关于不同浓度的镁对骨细胞的影响知之甚少。考虑到1 mM是培养细胞的细胞外镁的生理浓度，在我们的实验模型中，我们将成骨细胞如SaOS-2细胞和正常人成骨细胞暴露于低（0.1mM）和高（5.0mM）浓度的镁。我们发现，高浓度的镁显着抑制沉积的矿物基质的SaOS-2以及碱性磷酸酶，成骨细胞分化的标志物的活性。然后，我们通过测量碱性磷酸酶活性来评估正常人成骨细胞的分化，并再次发现高浓度的镁有明显的抑制作用。已知在骨形成中起作用的一氧化氮似乎不参与其中。我们推测，高水平的镁可能会改变细胞内各种阳离子的浓度-其中钙-通过竞争相同的转运蛋白。我们的结论是，高镁水平会损害成骨细胞活性，因此可能会导致骨骼疾病。
Several studies in humans indicate that both high and low concentrations of magnesium have harmful effects on bone metabolism and homeostasis. However, little is known about the effects of different concentrations of magnesium on bone cells. Considering that 1mM is the physiological concentration of extracellular magnesium for cultured cells, in our experimental model we exposed osteoblast like SaOS-2 cells and normal human osteoblasts to low (0.1mM) and high (5.0mM) concentrations of magnesium. We found that high concentrations of magnesium markedly inhibited the deposition of mineral matrix by SaOS-2 as well as the activity of alkaline phosphatase, a marker of osteoblast differentiation. We then evaluated the differentiation of normal human osteoblasts by measuring alkaline phosphatase activity and again found a marked inhibition by high concentrations of magnesium. Nitric oxide, which is known to play a role in bone formation, does not seem to be involved. We hypothesize that high levels of magnesium might alter the intracellular concentration of various cations - among which calcium - by competing for the same transporters. We conclude that high magnesium levels impair osteoblast activity and might therefore contribute to bone disease.