The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment
The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment
复制标题
采后病原菌扩展青霉生长和展青霉素产生对外源亚磷酸钾处理的响应
DOI：
10.1016/j.ijfoodmicro.2016.12.017
复制
发表时间：
2017-03-06
影响因子：
5.4
通讯作者：
Zhou, Ting
中科院分区：
文献类型：
--
作者：
Lai, Tongfei;Wang, Ying;Zhou, Ting
研究了外源亚磷酸钾（Phi）对采后病原菌扩展青霉（Penicillium expansum）生长和棒曲霉素产生的影响。结果表明，在5 mmol/L Phi胁迫下，扩展青霉生长发育明显受阻，棒曲霉素产量降低，对苹果果实的侵染性降低。同时，对15个与棒曲霉素生物合成相关的基因的表达分析表明，Phi主要在转录水平上影响棒曲霉素合成途径的早期步骤。此外，通过iTRAQ（用于相对和绝对定量的同量异序标签）和RNA-seq（RNA测序）方法评估了在6小时Phi胁迫期间扩展青霉孢子的蛋白质组和转录组改变的全局视图。共获得582个差异表达蛋白（DEPs）和177个差异表达基因（DEGs），其中大部分参与碳水化合物代谢、氨基酸代谢、脂类代谢、遗传信息处理和次生代谢产物的生物合成。最后，通过iTRAQ和RNA-seq数据集的相关性分析，筛选出39个重叠的候选序列。这些研究结果将为探讨Phi对扩展青霉生长和棒曲霉素生物合成的抑制机制提供更准确和有方向性的线索，并有助于开发基于Phi的有效控制方法。(C)2016爱思唯尔B. V.保留所有权利。
In this study, the effects of exogenous potassium phosphite (Phi) on growth and patulin production of postharvest pathogen Penicillium expansum were assessed. The results indicated that P. expansum under 5 mmol/L Phi stress presented obvious development retardation, yield reduction of patulin and lower infectivity to apple fruit. Meanwhile, expression analysis of 15 genes related to patulin biosynthesis suggested that Phi mainly affected the early steps of patulin synthetic route at transcriptional level. Furtherniore, a global view of proteome and transcriptome alteration of P. expansum spores during 6 h of Phi stress was evaluated by iTRAQ (isobaric tags for relative and absolute quantitation) and RNA-seq (RNA sequencing) approaches. A total of 582 differentially expressed proteins (DEPs) and 177 differentially expressed genes (DEGs) were acquired, most of which participated in carbohydrate metabolism, amino acid metabolism, lipid metabolism, genetic information processing and biosynthesis of secondary metabolites. Finally, 39 overlapped candidates were screened out through correlational analysis between iTRAQ and RNA-seq datasets. These findings will afford more precise and directional clues to explore the inhibitory mechanism of Phi on growth and patulin biosyhthesis of P. expansum, and be beneficial to develop effective controlling approaches based on Phi. (C) 2016 Elsevier B.V. All rights reserved.