Optical Biochip with Multichannels for Detecting Biotin-Streptavidin Based on Localized Surface Plasmon Resonance
Optical Biochip with Multichannels for Detecting Biotin-Streptavidin Based on Localized Surface Plasmon Resonance
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DOI：
10.1007/s11468-009-9094-2
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发表时间：
2009-09-01
期刊：
影响因子：
3
通讯作者：
Fu, Yongqi
中科院分区：
文献类型：
--
作者：
Zhu, Shaoli;Fu, Yongqi
高通量快速、准确地检测抗原抗体信号传导的分子结合对于生物传感技术非常重要。我们提出了一种新型多通道光学生物芯片，用于基于局域表面等离子体共振检测生物素-链霉亲和素。使用光刻技术制造光学生物芯片，以在玻璃基板上形成具有多通道功能的微阵列。每个微阵列中有不同的纳米结构。应用干法刻蚀和纳米球光刻技术来制造银纳米结构，如半球、纳米圆柱形、三角形和菱形纳米结构。我们证明，这些光学生物芯片上的 100 nM 目标分子（链霉亲和素）可以通过紫外-可见光谱仪轻松检测到。这表明纳米结构的周期和形状显着影响不同形状和几何参数的纳米结构的光学性能。我们的实验结果表明，具有多通道的光学生物芯片可以同时使用不同形状和周期结构的微阵列来检测目标分子。通过嵌入在同一芯片上的不同通道可以相应地实现对不同生物分子的免疫分析的批量处理。
A rapid and accurate detection of molecular binding of antigen-antibody signaling in high throughput is of great importance for biosensing technology. We proposed a novel optical biochip with multichannels for the purpose of detection of biotin-streptavidin on the basis of localized surface plasmon resonance. The optical biochip was fabricated using photolithography to form the microarrays functioning with multichannels on glass substrate. There are different nanostructures in each microarray. Dry etching and nanosphere lithography techniques were applied to fabricate Ag nanostructures such as hemispheres, nanocylindricals, triangular, and rhombic nanostructures. We demonstrated that 100-nM target molecule (streptavidin) on these optical biochips can be easily detected by a UV-visible spectrometer. It indicated that period and shape of the nanostructures significantly affect the optical performance of the nanostructures with different shapes and geometrical parameters. Our experimental results demonstrated that the optical biochips with the multichannels can detect the target molecule using the microarrays structured with different shapes and periods simultaneously. Batch processing of immunoassay for different biomolecular through the different channels embedded on the same chip can be realized accordingly.