Exposure to graphene oxide at environmental concentrations induces thyroid endocrine disruption and lipid metabolic disturbance in Xenopus laevis
Exposure to graphene oxide at environmental concentrations induces thyroid endocrine disruption and lipid metabolic disturbance in Xenopus laevis
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暴露于环境浓度的氧化石墨烯会导致非洲爪蟾的甲状腺内分泌紊乱和脂质代谢紊乱
DOI：
10.1016/j.chemosphere.2019.124834
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Wang Qiangwei
中科院分区：
文献类型：
--
作者：
Li Meng;Zhu Jiaping;Wang Mengcen;Fang Hua;Zhu Guonian;Wang Qiangwei
氧化石墨烯（GO）因其环境和健康风险而日益受到关注。然而，GO对野生动物的潜在毒性作用仍然有限。本研究选择非洲爪蟾作为模型，以评估甲状腺内分泌干扰以及脂质代谢紊乱的GO。在51阶段的蝌蚪暴露于GO（0，0.01，0.1和1 mg/L）21天，当蝌蚪正在经历一个非常复杂的形态变化和生长阶段。GO处理组表现出明显的发育毒性，表现为鼻口距和后肢长度缩短，体重下降，发育期延迟。暴露于GO也引起全身三碘甲状腺原氨酸（T3）和甲状腺素（T4）浓度的明显下降。与下丘脑-垂体-甲状腺（HPT）轴相关基因的mRNA表达也发生了显著变化。此外，我们观察到脂肪酸和甘油三酯（TG）的显着下降，伴随着GO暴露组中参与脂质合成和代谢的基因表达的变化。与此相反，高密度脂蛋白（HDL）和总胆汁酸水平显着增加，但胆固醇和低密度脂蛋白（LDH）水平无明显变化。以上结果首次揭示了GO可引起甲状腺内分泌紊乱，并对脂质合成和代谢产生明显的干扰作用。
Graphene oxide (GO) has become a topic of increasing concern for its environmental and health risks. However, the potential toxic effects of GO on wildlife remain limited. The present study chose theXenopus laevistadpole as a model to assess the thyroid endocrine disruption as well as the lipid metabolic disturbance of GO. Tadpoles at the 51 stage were exposed to GO (0, 0.01, 0.1, and 1 mg/L) for 21 days, when tadpoles were undergoing an extremely complicated phase of morphological changes and growth. GO treatment showed obvious developmental toxicity, such as shortened snout-to-vent length (SVL) and hind limb length (HLL), decreased body weight, and delayed developmental stage. Exposure to GO also induced obvious decreases in whole-body triiodothyronine (T3) and thyroxin (T4) concentrations. The mRNA expression of genes related to the hypothalamic–pituitary–thyroid (HPT) axis also changed significantly. Furthermore, we observed significant decline in the fatty acids and triglycerides (TGs) concomitantly with changes in the expression of genes involved in the synthesis and metabolism of lipids in GO exposure groups. In contrast, high-density lipoprotein (HDL) and total bile acid levels increased remarkably, but cholesterol and low-density lipoprotein (LDH) levels showed no obvious changes. Taken together, the results revealed for the first time that GO could induce thyroid endocrine disruption and produce obvious disturbance effect on lipid synthesis and metabolism.