SUPEROXIDE-DISMUTASE AND CATALASE REDUCE INFARCT SIZE IN A PORCINE MYOCARDIAL OCCLUSION-REPERFUSION MODEL
SUPEROXIDE-DISMUTASE AND CATALASE REDUCE INFARCT SIZE IN A PORCINE MYOCARDIAL OCCLUSION-REPERFUSION MODEL
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DOI：
10.1016/s0022-2828(86)80294-2
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发表时间：
1986-10-01
影响因子：
5
通讯作者：
OBERG, A
中科院分区：
文献类型：
--
作者：
NASLUND, U;HAGGMARK, S;OBERG, A
我们研究了在开胸闭塞再灌注模型中超氧化物歧化酶和过氧化氢酶是否可以减少心肌梗死面积。30头猪被用于实验。结扎左前降支60 min，再灌注5 h。在随机化和设盲后，将两种酶或安慰剂在闭塞前即刻和闭塞结束时作为推注注射到左心房中，并在再灌注期的第一个小时内作为连续输注注射。每种酶的总剂量为8 mg/kg bw。四氮唑染色测定梗死面积。在完成所有计算和排除之前，研究代码未破盲。9只动物死于顽固性室颤，最常见于闭塞期间。另外三个由于技术原因被排除在外。我们发现，超氧化物歧化酶和过氧化氢酶使危险心肌的梗死面积从平均89%减少到63%（P < 0.01）。推注输注后两种酶的初始血浆半衰期计算为30 min。
We investigated if superoxide dismutase and catalase could reduce myocardial infarct size in an open chest occlusion-reperfusion model. Thirty pigs were used for the experiment. The left anterior descending artery was ligated for 60 min followed by a 5 h reperfusion period. After randomisation and blinding the two enzymes or placebo were injected into the left atrium as a bolus immediately before and at the end of the occlusion and as a continuous infusion over the first hour of the reperfusion period. The total dose for each enzyme was 8 mg/kg bw. Tetrazolium staining was used to determine infarct size. The study code was not broken untill all calculations and exclusions had been made. Nine animals died from intractable ventricular fibrillation, most commonly during the occlusion. Another three were excluded for technical reasons. We found that superoxide dismutase and catalase reduced infarct size in relation to myocardium at risk from a mean of 89% to 63% (P < 0.01). Initial plasma half life for the two enzymes after the bolus infusions were calculated to be 30 min.