Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers.
Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers.
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DOI：
10.1038/srep23722
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发表时间：
2016-03-30
影响因子：
4.6
通讯作者：
Verkhovsky AB
中科院分区：
文献类型：
--
作者：
Labouesse C;Gabella C;Meister JJ;Vianay B;Verkhovsky AB
Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the cytoplasm. By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous properties.
