Anti-Inflammatory Activity of Curcumin in Macrophages Stimulated by Lipopolysaccharides from Porphyromonas gingivalis
Anti-Inflammatory Activity of Curcumin in Macrophages Stimulated by Lipopolysaccharides from Porphyromonas gingivalis
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DOI：
10.1159/000161127
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发表时间：
2008-01-01
期刊：
影响因子：
3.1
通讯作者：
Bian, Zhuan
中科院分区：
文献类型：
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作者：
Chen, Dong;Nie, Min;Bian, Zhuan
背景：牙龈卟啉单胞菌是一种主要的牙周病致病菌，是牙周炎发生的必要条件。牙龈假单胞菌的脂多糖刺激免疫细胞分泌细胞因子，从而引发与牙周炎相关的炎症。巨噬细胞是牙周炎症部位的重要免疫细胞。姜黄素是一种在香料姜黄中发现的主要姜黄素类化合物，具有抗炎特性。本研究旨在探讨姜黄素对内毒素刺激的巨噬细胞的抗炎作用及其机制。方法：用不同浓度的姜黄素处理RAW264.7细胞，用牙龈假单胞菌内毒素刺激RAW264.7细胞。逆转录-聚合酶链式反应和酶联免疫吸附试验分别检测肿瘤坏死因子-α和IL-1β的表达。然后，用荧光素酶分析法检测核因子-kappaB依赖转录的激活情况。结果：姜黄素呈剂量依赖性地抑制牙龈假单胞菌刺激的RAW264.7细胞的TNF-α和IL-1β基因表达及蛋白合成。牙龈假单胞菌脂多糖激活RAW264.7细胞中依赖于核因子-kappaB的转录，姜黄素也可下调该转录。结论：姜黄素可抑制牙龈假单胞菌内毒素诱导的细胞因子表达，其机制可能与抑制核因子-kappaB途径有关。版权所有(C)2008 S.Karger AG，巴塞尔。
Background: Porphyromonas gingivalis, a major periodontopathic bacterium, is necessary for periodontitis to take place. The lipopolysaccharide (LPS) of P. gingivalis stimulates cytokine secretion in immune cells, and thereby initiates the inflammation related to periodontitis. Macrophages are the important ones of the immune cells that are prominent at inflammatory periodontal sites. Curcumin, a major curcumanoid found in the spice turmeric, exhibits anti-inflammatory properties. The aim of this study was to investigate the anti-inflammatory effect and the mechanism of action of curcumin in macrophages stimulated by P. gingivalis LPS. Methods: RAW264.7 cells pre-treated with various concentrations of curcumin were stimulated by P. gingivalis LPS. TNF-alpha and IL-1 beta expressions were separately detected by RT-PCR and ELISA. Next, activation of NF-kappa B-dependent transcription was examined by luciferase assay. Results: Curcumin dose-dependently inhibited TNF-alpha and IL-1 beta gene expression and protein synthesis in RAW264.7 cells stimulated with P. gingivalis LPS. P. gingivalis LPS activated NF-kappa B-dependent transcription in RAW264.7 cells, which were down-regulated by pre-treatment with curcumin as well. Conclusion: Our data suggest that curcumin can inhibit P. gingivalis LPS-induced cytokine expression, and that this could be due to the inhibition of the NF-kappa B pathway. Copyright (C) 2008 S. Karger AG, Basel.