Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis
Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis
复制标题
DOI：
10.1038/emboj.2008.112
复制
发表时间：
2008-07-09
期刊：
影响因子：
11.4
通讯作者：
Prekeris, Rytis
中科院分区：
文献类型：
--
作者：
Simon, Glenn C.;Schonteich, Eric;Prekeris, Rytis
胞质分裂是一个高度调节和动态的事件，涉及细胞骨架和膜室的重组。最近，FIP 3已经涉及靶向再循环内体到分裂细胞的中间体，并且发现是分裂所需的。在这里，我们证明，centralspindlin组件Cyk-4是一个FIP 3结合蛋白。此外，我们表明，FIP 3结合Cyk-4在晚期末期和centralspindlin可能需要FIP 3招聘到中间体。我们绘制了FIP 3结合区Cyk-4，并表明它与ECT 2结合结构域重叠。最后，我们证明，FIP 3和ECT 2形成相互排斥的复合物与Cyk-4和ECT 2的解离从中间体在后期末期可能需要的FIP 3和再循环的内体的分裂沟的招聘。因此，我们提出centralspindlin复合物不仅调节肌动蛋白-肌球蛋白环收缩，而且还调节内吞囊泡运输到卵裂沟，并且它通过与ECT 2和FIP 3的顺序相互作用来实现。
Cytokinesis is a highly regulated and dynamic event that involves the reorganization of the cytoskeleton and membrane compartments. Recently, FIP3 has been implicated in targeting of recycling endosomes to the mid-body of dividing cells and is found required for abscission. Here, we demonstrate that the centralspindlin component Cyk-4 is a FIP3-binding protein. Furthermore, we show that FIP3 binds to Cyk-4 at late telophase and that centralspindlin may be required for FIP3 recruitment to the mid-body. We have mapped the FIP3-binding region on Cyk-4 and show that it overlaps with the ECT2-binding domain. Finally, we demonstrate that FIP3 and ECT2 form mutually exclusive complexes with Cyk-4 and that dissociation of ECT2 from the mid-body at late telophase may be required for the recruitment of FIP3 and recycling endosomes to the cleavage furrow. Thus, we propose that centralspindlin complex not only regulates acto-myosin ring contraction but also endocytic vesicle transport to the cleavage furrow and it does so through sequential interactions with ECT2 and FIP3.