Bacillus subtilis operon under the dual control of the general stress transcription factor sigma B and the sporulation transcription factor sigma H.
Bacillus subtilis operon under the dual control of the general stress transcription factor sigma B and the sporulation transcription factor sigma H.
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枯草芽孢杆菌操纵子在一般胁迫转录因子 sigma B 和孢子形成转录因子 sigma H 的双重控制下。
DOI：
10.1111/j.1365-2958.1996.tb02621.x
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发表时间：
1996
影响因子：
3.6
通讯作者：
Price,CW
中科院分区：
文献类型：
--
作者：
Varón,D;Brody,MS;Price,CW
枯草芽孢杆菌的σB转录因子在各种环境胁迫下被激活，包括进入生长稳定期施加的压力，以及对对数生长的细胞进行热、盐或乙醇挑战。尽管σB被认为控制着一般的应力调节，但它所指导的细胞功能范围在很大程度上仍然是未知的。我们理解σB的生理作用的方法是描述需要该因子全部或部分表达的基因，即sb基因。在本研究中，我们报道了转座子插入csb40：：Tn917lac3个开放阅读框的操纵子，其中第二个类似于干旱胁迫诱导的植物蛋白。引物延伸和操纵子融合实验表明，ecsb40操纵子有一个依赖于σB的启动子，该启动子在对数生长的细胞中被盐强烈诱导。Secsb40操纵子还有第二个σH依赖的启动子，不受盐添加的影响。这些结果支持了σ带控制一个一般应力调节子的假说，并表明σ带σHRegons部分重叠。我们认为，除了它在孢子形成过程中的公认作用外，σHis还参与控制广泛参与一般应激反应的基因的一个亚类。
The σBtranscription factor ofBacillus subtilisis activated in response to a variety of environmental stresses, including those imposed by entry into the stationary‐growth phase, and by heat, salt or ethanol challenge to logarithmically growing cells. Although σBis thought to control a general stress regulon, the range of cellular functions it directs remains largely unknown. Our approach to understand the physiological role of σBis to characterize genes that require this factor for all or part of their expression, i.e. thecsbgenes. In this study, we report that the transposon insertioncsb40::Tn917lacidentifies an operon with three open reading frames, the second of which resembles plant proteins induced by desiccation stress. Primer‐extension and operon‐fusion experiments showed that thecsb40operon has a σB‐dependent promoter which is strongly induced by the addition of salt to logarithmically growing cells. Thecsb40operon also has a second, σH‐dependent promoter that is unaffected by salt addition. These results provide support for the hypothesis that σBcontrols a general stress regulon, and indicate that the σBand σHregulons partly overlap. We suggest that in addition to its acknowledged role in the sporulation process, σHis also involved in controlling a subclass of genes that are broadly involved in a general stress response.