Effects of bufalin and cinobufagin on the proliferation of androgen dependent and independent prostate cancer cells
Effects of bufalin and cinobufagin on the proliferation of androgen dependent and independent prostate cancer cells
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DOI：
10.1002/pros.10172
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发表时间：
2003-02-01
期刊：
影响因子：
2.8
通讯作者：
Wang, PS
中科院分区：
文献类型：
--
作者：
Yeh, JY;Huang, WJ;Wang, PS
背景强心苷可诱导癌症的溶瘤作用。本研究旨在探讨蟾蜍灵和华蟾素对前列腺癌细胞株LNCaP、DU 145和PC 3增殖的影响。MTT法检测细胞增殖;通过乳酸脱氢酶测定确定细胞毒性作用。用双波长分光光度计测定细胞内钙离子浓度（[Ca ~（2+）]（i））。TUNEL法和流式细胞术检测凋亡细胞百分率。比色法测定半胱天冬酶活性。蟾毒灵和华蟾毒精在0.1、1或10 μ M剂量下培养2-4天后抑制癌细胞增殖。蟾毒灵和华蟾毒精对DU 145和LNCaP细胞的细胞毒性具有剂量依赖性。蟾毒灵和华蟾素可增加培养24 h后癌细胞内[Ca 2 +]（i）和凋亡，增加DU 145和PC 3细胞caspase 3活性和LNCaP细胞caspase 9活性。蟾毒灵和华蟾毒精可抑制前列腺癌细胞系的增殖，与[Ca 2 +]（i）和凋亡的持续升高相关。(C)2002 Wiley-Liss，Inc.
BACKGROUND. Cardiac glycosides may induce oncolytic effects in cancers. This study was to evaluate bufalin and cinobufagin effects on the proliferation of prostate cancer cell lines named LNCaP, DU145, and PC3.METHODS. Cell proliferation was measured by MTT assay. The cytotoxic effects were determined by lactate clehydrogenase measurements. The intracellular calcium concentration ([Ca2+](i)) was measured by a dual-wavelength spectrometer system. TUNEL assay and flow cytometry were performed to measure percentage of apoptotic cells. A colorimetric assay was to measure caspases activities.RESULTS. Bufalin and cinobufagin inhibited proliferation of cancer cells at doses of 0.1, 1, or 10 muM after 2-4 days of culture. Cytotoxicity of bufalin and cinobufagin on the DU145 and LNCaP cells was dose-dependent. Bufalin or cinobufagin increased [Ca2+](i) and apoptosis in cancer cells after a 24-hr culture as well as caspase 3 activities in DU145 and PC3 cells and caspase 9 activities in LNCaP cells.CONCLUSIONS. Bufalin and cinobufagin may inhibit the proliferation of prostate cancer cell lines associated with sustained elevation of the [Ca2+](i) and that of apoptosis. (C) 2002 Wiley-Liss, Inc.