Xenopus A-myb is expressed during early spermatogenesis.
Xenopus A-myb is expressed during early spermatogenesis.
复制标题
非洲爪蟾 A-myb 在早期精子发生过程中表达。
作者：
J. Sleeman
c-myb已被深入研究，但myb基因家族的其他成员在很大程度上被忽视。本文描述了非洲爪蟾的cDNA序列，XAMyb，密切相关的人类A-myb的分离和鉴定。非洲爪蟾为研究基因参与细胞增殖、分化和发育提供了一个有价值的实验模型。尽管在卵母细胞或卵子中或在早期胚胎发生期间未检测到XAMyb的表达，但在卵巢组织内存在低水平的表达，其响应于刺激卵母细胞成熟的激素而下调。然而，在成年雄性非洲爪蟾中，几乎所有的A-myb表达都在睾丸中，特别是在精原细胞的细胞核内，精原细胞是精子的有丝分裂增殖的早期祖细胞。表达福尔斯显着下降，在减数分裂，并在随后的精子发生过程中几乎检测不到。非洲爪蟾A-myb在生殖细胞发育的前终末分化/增殖阶段的表达模式类似于造血过程中类似阶段的c-myb表达。这些结果表明myb变异体在不同干细胞衍生群体发育过程中的相关作用。
c-myb has been studied intensely, but other members of the myb gene family have been largely overlooked. This paper describes the isolation and characterization of a Xenopus cDNA sequence, XAMyb, closely related to human A-myb. Xenopus provides a valuable experimental model for investigating the involvement of genes in cell proliferation, differentiation and development. Although no expression of XAMyb was detected in oocytes or eggs or during early embryogenesis, there is a low level of expression within ovarian tissue, which is down-regulated in response to hormones that stimulate oocyte maturation. However, in adult male Xenopus, virtually all A-myb expression is in the testis, particularly within the nuclei of spermatogonial cells, the mitotically proliferating early progenitors of spermatozoa. Expression falls dramatically during meiosis and is virtually undetectable during subsequent spermiogenesis. This pattern of expression of Xenopus A-myb during the preterminal differentiation/proliferation phase of germ cell development resembles the expression of c-myb in similar phases during haematopoiesis. These results suggest related roles for myb variants during the development of different stem cell-derived populations.