Research on the establishment of chronic stress-induced premature ovarian failure the rat model and effects of Chinese medicine Muniziqi treatment
Research on the establishment of chronic stress-induced premature ovarian failure the rat model and effects of Chinese medicine Muniziqi treatment
复制标题
慢性应激性卵巢早衰大鼠模型的建立及中药孟子芪治疗效果研究
DOI：
10.1002/mrd.23092
复制
发表时间：
2019-02-01
影响因子：
2.5
通讯作者：
Yilike, Xiamixinuer
中科院分区：
文献类型：
--
作者：
Keremu, Abulizi;Yaoliwasi, Aziguli;Yilike, Xiamixinuer
本研究的目的是建立慢性应激性卵巢早衰(POF)动物模型，并探讨其生物学基础及中药目子气的治疗作用。将性成熟雌性SD大鼠在寒冷和潮湿的条件下用菠菜和香菜喂养约20周，直到建立慢性应激(CS)模型。根据每周生物学特性和激素水平检测(黄体生成素、卵泡刺激素、雌激素)，将CS大鼠分为POF应激模型组和应激模型组。将POF病应激模型组分为高、中、低药物干预组，观察慕尼滋气的治疗作用。结果表明，慢性应激(特殊食物、寒冷、潮湿)可导致POF病。中药目子气除能改善生殖激素水平紊乱外，还能改善下丘脑-垂体-卵巢轴的功能。其机制可能是通过改变血清中雌二醇、促黄体生成素和卵泡刺激素水平，降低卵巢早衰相关蛋白PFN-1的表达。
The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in cold and wet conditions for about 20 weeks until a chronic stress (CS) model was established. The CS rats were divided into a POF stress model group and a stress model group according to weekly biological characteristics and hormone level detection ( luteinizing hormone [LH], follicle stimulating hormone [FSH], and estrogen [E2]). To investigate the therapeutic effect of Muniziqi, the POF disease stress model group was divided into the high-, medium-, and low-drug intervention groups. The results showed that chronic stresses (special food, cold, damp) can lead to POF disease. The traditional Chinese medicine Muniziqi could not only improve the reproductive hormone level disorder, but also improve the function of the hypothalamus-pituitary-ovarian axis. The underlying mechanism may be a change in the E2, LH, and FSH hormone levels in serum and lower expression of ovarian premature aging-related protein PFN-1.