Directing neuronal cell growth on implant material surfaces by microstructuring
Directing neuronal cell growth on implant material surfaces by microstructuring
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DOI：
10.1002/jbm.b.32656
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发表时间：
2012-05-01
影响因子：
3.4
通讯作者：
Reuter, Guenter
中科院分区：
文献类型：
--
作者：
Reich, Uta;Fadeeva, Elena;Reuter, Guenter
为了获得最佳的听觉感觉，听觉假体的电极必须与相应的神经元细胞具有最佳的电接触。为了改善电极神经界面，植入物表面的微结构可以引导神经元细胞朝向电极接触。为此，采用飞秒激光烧蚀在目前相关的两种人工耳蜗植入材料——硅酮弹性体和铂——上生成线性微槽。有机硅表面通过两种不同的方法进行结构化，或者直接通过激光烧蚀，或者间接通过使用激光微结构化模具压印。利用神经元样细胞系和原代听觉神经元研究了表面结构对神经突生长的影响。在微结构听觉植入材料上培养嗜铬细胞瘤细胞系 PC-12 和原代螺旋神经节细胞。确定了神经突生长相对于微纹的方向。两种细胞类型都显示出与铂和模制有机硅弹性体上的微结构平行的优选取向。有趣的是，直接激光烧蚀硅树脂产生的微观结构不会影响任何一种细胞类型的方向。这表明制造程序的差异会影响微结构植入物表面引导神经突生长的能力。这对于临床应用尤其重要，因为成型技术代表了医疗植入物微结构有机硅表面的可重复、经济且商业上可行的制造程序。 (c) 2012 年 Wiley periodicals, Inc. J Biomed Mater Res B 部分：Appl Biomater，2012。
For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrodenerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater , 2012.