CHANGES IN CU,ZN-SUPEROXIDE DISMUTASE, CYTOCHROME-C-OXIDASE, GLUTATHIONE-PEROXIDASE AND GLUTATHIONE TRANSFERASE ACTIVITIES IN COPPER-DEFICIENT MICE AND RATS
CHANGES IN CU,ZN-SUPEROXIDE DISMUTASE, CYTOCHROME-C-OXIDASE, GLUTATHIONE-PEROXIDASE AND GLUTATHIONE TRANSFERASE ACTIVITIES IN COPPER-DEFICIENT MICE AND RATS
复制标题
DOI：
10.1093/jn/121.3.355
复制
发表时间：
1991-03-01
影响因子：
4.2
通讯作者：
PROHASKA, JR
中科院分区：
文献类型：
--
作者：
PROHASKA, JR
在瑞士白化小鼠和SpragueDawley大鼠中造成饮食铜缺乏，以比较选定的抗氧化酶的变化。采用5wk饮食治疗，小鼠出生后约1wk(最初通过水坝)，大鼠出生后约3wk。使用断奶后范式对小鼠进行了另一项验证性实验。对小鼠(6周龄)和大鼠(8周龄)的子代进行铜缺乏处理，并与铜充足的对照组进行比较。与适铜动物相比，缺铜小鼠和大鼠贫血，铜蓝蛋白活性和肝脏铜含量较低，心脏和小肠相对重量较高。低铜动物的细胞色素c氧化酶(小鼠)和铜锌超氧化物歧化酶(小鼠和大鼠)的活性均低于铜充足动物，但存在器官和物种差异。与适铜对照组相比，缺铜小鼠和大鼠肝脏和血浆中谷胱甘肽过氧化物酶活性降低。在1周龄开始治疗的缺铜小鼠中，肝脏谷胱甘肽转移酶活性显着降低，但在断奶后缺铜的小鼠或大鼠中则不明显。
Dietary copper deficiency was produced in Swiss albino mice and Sprague Dawley rats to compare changes in selected antioxidant enzymes. A 5-wk dietary treatment was employed, starting approximately 1 wk after birth for mice (initially via dams) and 3 wk after birth for rats. An additional confirmatory experiment was conducted with mice using the postweanling paradigm. Mouse offspring (6 wk of age) and rats (8 wk of age) maintained on a Cu-deficient treatment were compared with Cu-adequate controls. Compared with Cu-adequate animals, Cu-deficient mice and rats were anemic, had lower ceruloplasmin activities and liver copper levels, and had higher relative weights of heart and small intestine. Activity of cytochrome c oxidase (mice) and Cu,Zn-su-peroxide dismutase (mice and rats) was lower in all seven organs examined from Cu-deficient animals compared with Cu-adequate animals, although there were organ and species differences. Compared with Cu-adequate controls, glutathione peroxidase activity was lower in liver and plasma of Cu-deficient mice and rats. Hepatic glutathione transferase activity was markedly lower in those Cu-deficient mice started on treatment at 1 wk of age but not in those mice or rats subjected to postweanling copper deficiency.