Nuclear insertion sequences of mitochondrial DNA predominate in hair but not in blood of elephants

Nuclear insertion sequences of mitochondrial DNA predominate in hair but not in blood of elephants
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DOI:
10.1046/j.1365-294x.1999.00507.x
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发表时间:
1999-01-01
期刊:
影响因子:
4.9
通讯作者:
Pääbo, S
Pääbo, S
中科院分区:
生物学1区
文献类型:
--
作者:
Greenwood, AD;Pääbo, S

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头发已成为群体遗传学、法医学和保护生物学中广泛使用的 DNA 来源。在这里,我们报道了从血液 DNA 中扩增线粒体控制区片段的 PCR 引物主要从毛发 DNA 中扩增线粒体 DNA 的整合核拷贝。因此,在某些物种中,在某些情况下,头发中的 DNA 可能会产生不可靠的结果。
Hair has become a widely used source of DNA in population genetics, forensics, and conservation biology. Here we report that PCR primers that amplify a segment of the mitochondrial control region from blood DNA amplify primarily integrated nuclear copies of mitochondrial DNA from hair DNA. Thus, in some species, and under some circumstances, DNA from hair may yield unreliable results.