Identification of a long non-coding RNA NR_026689 associated with lung carcinogenesis induced by NNK.
Identification of a long non-coding RNA NR_026689 associated with lung carcinogenesis induced by NNK.
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与 NNK 诱导肺癌发生相关的长非编码 RNA NR_026689 的鉴定
DOI：
10.18632/oncotarget.7475
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发表时间：
2016-03-22
期刊：
影响因子：
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通讯作者：
Jiang Y
中科院分区：
文献类型：
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作者：
Wu J;Li X;Xu Y;Yang T;Yang Q;Yang C;Jiang Y
长链非编码RNA（lncRNA）被认为是参与多种癌症发展的重要表观遗传调节因子。lncRNA表达的改变与暴露于化学致癌物有关。然而，肺癌发生过程中lncRNA的表达是否由化学致癌物诱导尚不清楚。在本研究中，使用我们先前研究建立的NNK诱导的大鼠肺癌模型，我们测定了lncRNA表达谱，并且在NNK治疗组的肺癌组织和血液中观察到lncRNA表达的改变。采用定量逆转录PCR（qRT-PCR），进一步检测并验证了5个差异表达的lncRNA。我们鉴定了一种新的lncRNA，NR_026689，其显示在NNK诱导的肺癌组织中表达增加，并且在肺组织中特异性观察到lncRNA NR_026689的改变。测定NR_026689的水平，并在NNK治疗后第10周和第20周在大鼠全血中显著增加，以评估其作为肺癌的潜在早期标志物。总之，这些发现表明lncRNA NR_026689可能是肺癌的潜在早期生物标志物，并且与NNK诱导的肺癌发生相关。
Long non-coding RNAs (lncRNA) are thought to be important epigenetic regulators involved in the development of a variety of cancers. Alterations in lncRNA expression are associated with exposure to chemical carcinogens. However, it is still unclear whether lncRNA expression during lung carcinogenesis is induced by chemical carcinogens. In this study, using NNK-induced rat lung cancer model established by our previous study, we determined the lncRNA expression profiles, and an alteration in lncRNA expression was observed in lung cancer tissues and blood in the NNK treatment group. Using quantitative reverse-transcription PCR (qRT-PCR), five differentially expressed lncRNAs were further detected and validated. We identified a novel lncRNA, NR_026689, which showed increased expression in lung cancer tissues induced by NNK and the alteration of lncRNA NR_026689 was specifically observed in lung tissue. The level of NR_026689 was determined and significantly increased in rat whole blood at the 10th and 20th week after NNK treatment to evaluate it as a potential early marker for lung cancer. Together, these findings suggest that lncRNA NR_026689 may be a potential early biomarker for lung cancer and is associated with lung carcinogenesis induced by NNK.