Three-Layered PCL Grafts Promoted Vascular Regeneration in a Rabbit Carotid Artery Model
Three-Layered PCL Grafts Promoted Vascular Regeneration in a Rabbit Carotid Artery Model
复制标题
三层 PCL 移植物促进兔颈动脉模型中的血管再生。
DOI：
10.1002/mabi.201500355
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发表时间：
2016
影响因子：
4.6
通讯作者：
Kong Deling
中科院分区：
文献类型：
--
作者：
Wang Kai;Zheng Wenting;Pan Yiwa;Ma Shaoyang;Guan Yong;Liu Ruming;Zhu Meifeng;Zhou Xin;Zhang Jun;Zhao Qiang;Zhu Yan;Wang Lianyong;Kong Deling
在这项研究中，三层聚（ε-己内酯）（PCL）接枝（tPCL）制备的静电纺丝PCL和静电喷涂聚（环氧乙烷）（PEO），它具有薄的致密内层，松散的中间层，和致密的外层。仅具有致密层的常规PCL移植物（rPCL）用作对照。在兔颈动脉中进行体内评价。在tPCL移植物壁内观察到增强的细胞浸润、内皮和平滑肌层的快速再生以及增加的弹性蛋白沉积。3个月后，tPCL移植物显示出比rPCL移植物更快的PCL降解。tPCL移植物中浸润的巨噬细胞分泌更高水平的单核细胞趋化蛋白-1（MCP-1）和血管内皮生长因子（VEGF），从而促进血管再生。结论：tPCL移植物可能是一种有用的血管假体，值得进一步研究。
In this study, a three layered poly (ε‐caprolactone) (PCL) graft (tPCL) was fabricated by electrospinning PCL and electrospraying poly (ethylene oxide) (PEO), which has a thin dense inner layer, a loose middle layer, and a dense outer layer. Regular PCL grafts (rPCL) with only a dense layer were used as control. In vivo evaluation was performed in rabbit carotid artery. Enhanced cell infiltration, rapid regeneration of endothelium and smooth muscle layers, and increased elastin deposition were observed within the tPCL graft wall. After 3 months, tPCL grafts showed faster PCL degradation than the rPCL grafts. Infiltrated macrophages in the tPCL grafts secreted higher level of monocyte chemoattractant protein‐1 (MCP‐1) and vascular endothelial growth factor (VEGF) which enhanced vascular regeneration. In conclusion, the tPCL graft may be a useful vascular prosthesis and worth for further investigation.