DETECTION OF A MUCIN MARKER FOR THE ADENOMA-CARCINOMA SEQUENCE IN HUMAN COLONIC MUCOSA BY MONOCLONAL-ANTIBODY AM-3
DETECTION OF A MUCIN MARKER FOR THE ADENOMA-CARCINOMA SEQUENCE IN HUMAN COLONIC MUCOSA BY MONOCLONAL-ANTIBODY AM-3
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DOI：
10.1136/jcp.43.5.379
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发表时间：
1990-05-01
影响因子：
3.4
通讯作者：
RIECKEN, EO
中科院分区：
文献类型：
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作者：
HANSKI, C;BORNHOEFT, G;RIECKEN, EO
单克隆抗体 AM-3 是针对从人结直肠癌中提取的粘蛋白而产生的。它与石蜡包埋的结直肠癌切片反应强烈。在结肠腺瘤组织中，表达 AM-3 检测到的表位的细胞百分比与不典型增生的程度相关。与免疫组织化学染色不同，免疫组织化学染色未显示组织学正常粘膜中存在抗原，而更灵敏的酶联免疫吸附测定（ELISA）和免疫印迹测定显示该抗原在该组织中表达较弱。 AM-3与几种正常和恶性人体组织发生不同频率的反应，表明检测到的表位不限于结肠组织。在结肠癌中，它存在于表观相对分子质量超过 440,000 的唾液酸粘蛋白上。这些数据表明，AM-3 可检测到的抗原可能有助于评估结肠腺瘤的癌前变化。
The monoclonal antibody AM-3 was raised against mucins extracted from human colorectal carcinomas. It reacted strongly with sections of paraffin wax embedded colorectal carcinoma. In colonic adenoma tissue the percentage of cells expressing the epitope detected by AM-3 correlated with the degree of dysplasia. In contrast to immunohistochemical staining, which did not show the presence of the antigen in histologically normal mucosa, the more sensitive enzyme linked immunosorbent assay (ELISA) and immunoblot assays showed that it was weakly expressed in this tissue. AM-3 reacted with variable frequency with several normal and malignant human tissues, indicating that the detected epitope is not restricted to colonic tissue. In colonic carcinomas it is present on a sialomucin of apparent relative molecular mass of more than 440,000. These data suggest that the antigen detectable with AM-3 may be useful in the assessment of premalignant changes in colonic adenomas.