Electrospun nanofibers as a tool for architecture control in engineered cardiac tissue
Electrospun nanofibers as a tool for architecture control in engineered cardiac tissue
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DOI：
10.1016/j.biomaterials.2011.04.042
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发表时间：
2011-08-01
期刊：
影响因子：
14
通讯作者：
Agladze, Konstantin
中科院分区：
文献类型：
--
作者：
Orlova, Yuliya;Magome, Nobuyuki;Agladze, Konstantin
本文提出了一种体外心脏组织工程系统的基础上培养的心肌细胞上静电纺丝聚甲基戊二酰亚胺（PMGI）纳米纤维网，无论是打印在固体基质或悬浮在空间。特别注意控制组织结构的能力。静电纺丝过程允许以随机或对齐的方式收集具有不同定位密度的纳米级直径PMGI纤维，其限定了网的一般配置。当纳米纤维之间的距离为30 μ M或更小时，微压印在固体基底上的纳米纤维保证对齐的细胞生长。悬浮在3D空间中的纳米纤维根据纳米纤维的取向和定位密度定义了组织的整体结构。结果，心肌细胞增殖成可收缩的组织丝、开放的组织网和连续的各向异性细胞片。细胞的排列通过细胞形状的伸长和由FFT数据支持的α-肌动蛋白丝的取向来表征。这种方法的优点是能够保持三维性和结构各向异性。(C)2011爱思唯尔有限公司版权所有。
This paper presents an in vitro system for cardiac tissue engineering based on cardiomyocytes cultured on electrospun polymethylglutarimide (PMGI) nanofibrous meshes either imprinted on solid substrate or suspended in space. Special care was taken over the ability to control the tissue architecture. The electrospinning process allowed nano-scale diameter PMGI fibers with different positioning density to be collected in a random or in an aligned way that defines the general configuration of the mesh. Micro-imprinted on solid substrate nanofibers guarantee aligned cell growth, when the distance between them is 30 mu M or less. Suspended in 3D space, nanofibers define the overall architecture of the tissue, depending on orientation and positioning density of the nanofibers. As a result, cardiac cells proliferated into contractile tissue filaments, open-worked tissue meshes and continuous anisotropic cell sheets. Alignment of the cells was characterized by elongation of the cell shape and orientation of the a-actin filaments supported by the FFT data. The advantage of this method is its ability to maintain both three-dimensionality and structural anisotropy. (C) 2011 Elsevier Ltd. All rights reserved.