Acute phase response in zebrafish upon Aeromonas salmonicida and Staphylococcus aureus infection:: Striking similarities and obvious differences with mammals
Acute phase response in zebrafish upon Aeromonas salmonicida and Staphylococcus aureus infection:: Striking similarities and obvious differences with mammals
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DOI：
10.1016/j.molimm.2006.03.001
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发表时间：
2007-01-01
影响因子：
3.6
通讯作者：
Xu, Anlong
中科院分区：
文献类型：
--
作者：
Lin, Bin;Chen, Shangwu;Xu, Anlong
斑马鱼已成为免疫学研究的一种有价值的模型。然而，对于其对传染性病原体的免疫反应的全貌知之甚少。在此，我们首次对其分别针对鲑鱼气单胞菌（一种革兰氏阴性菌）和金黄色葡萄球菌（一种革兰氏阳性菌）的免疫反应进行了系统研究。通过抑制性消减杂交鉴定了感染后诱导的基因，其中许多基因编码急性期蛋白（APPs）。与哺乳动物相比，观察到了显著的相似性和明显的差异。鉴定出了相似的急性期蛋白（血清淀粉样蛋白A、铁调素和触珠蛋白等）以及一个相似的急性期蛋白诱导系统（涉及Toll样受体、促炎细胞因子和C/EBP转录因子），这意味着鱼类和哺乳动物之间存在进化上保守的机制。还发现了一些新的急性期蛋白，这表明鱼类采用了不同的免疫策略。其中，白细胞衍生趋化因子2（LECT2）在感染后诱导倍数高达1000倍，为该基因的功能研究提供了新的线索。我们的研究结果首次证明了斑马鱼中存在相似但又不同的免疫反应，为研究先天免疫的进化保守机制和鱼类特异性机制开辟了新的途径。（c）2006爱思唯尔有限公司。保留所有权利。
Zebrafish has emerged as a valuable model for immunological studies. However, little is known about the overall picture of its immune response to infectious pathogens. Here we present the first systematic study of its immune response to Aeromonas salmonicida and Staphylococcits aureits, a Gram-negative and a Gram-positive bacteria, respectively. Genes induced upon infection were identified with suppression subtractive hybridization, with many of them encoding acute phase proteins (APPs). When compared with mammals, striking similarities and obvious differences have been observed. Both similar APPs (SAA, hepcidin and haptoglobin, etc.) and a similar system for the induction of APPs (which involves the TLRs, pro-inflammatory cytokines and C/EBPs) were identified, implying evolutionary conserved mechanisms among fish and mammals. Some novel APPs were also discovered. suggesting different immune strategies adopted by fish species. Among which, LECT2 was induced by up to 1000-fold upon infection, shedding new lights on the function of this gene. Our results constitute the first demonstration of a similar while different immune response in zebrafish and open new avenues for the investigation of evolutionary conserved and fish specific mechanisms of innate immunity. (c) 2006 Elsevier Ltd. All rights reserved.