Commercial simian virus antisera that inhibit virus replication in primary monkey kidney cell cultures.
Commercial simian virus antisera that inhibit virus replication in primary monkey kidney cell cultures.
复制标题
商业猴病毒抗血清，可抑制原代猴肾细胞培养物中的病毒复制。
DOI：
10.1128/jcm.13.5.824-827.1981
复制
发表时间：
1981
影响因子：
9.4
通讯作者：
Hamparian,VV
中科院分区：
文献类型：
--
作者：
Hughes,JH;Hamparian,VV
在细胞培养液中掺入高免疫血清以控制原代细胞的内源性病毒感染可以对其他病毒的复制产生显著影响。用市售猴病毒5或猴病毒40抗血清与原代猴肾细胞培养时，我们发现对副流感病毒2型、3型和呼肠孤病毒1型的复制有明显的抑制作用(大于90%)。在病毒诊断实验室，如果不先用高免疫血清清洗原代猴肾细胞，使用高免血清可能导致某些病毒的分离失败。
The incorporation of hyperimmune serum into cell culture medium to control endogenous viral infections of primary cells can have a significant effect on the replication of other viruses. When commercial simian virus 5 or simian virus 40 antiserum was used with primary monkey kidney cell cultures, we found a significant inhibition (greater than 90%) of the replication of parainfluenza virus types 2 and 3 and reovirus type 1. In the viral diagnostic laboratory, the use of hyperimmune serum with primary monkey kidney cells may result in failure to isolate certain viruses if these cells are not first washed free of hyperimmune serum.