CPTA modulates levels of carotenogenic proteins and their mRNAs and affects carotenoid and ABA content as well as chromoplast structure in Narcissus pseudonarcissus flowers
CPTA modulates levels of carotenogenic proteins and their mRNAs and affects carotenoid and ABA content as well as chromoplast structure in Narcissus pseudonarcissus flowers
复制标题
DOI：
10.1111/j.1438-8677.1999.tb00270.x
复制
发表时间：
1999-11-01
期刊：
影响因子：
3.9
通讯作者：
Beyer, P
中科院分区：
文献类型：
--
作者：
Al-Babili, S;Hartung, W;Beyer, P
为了研究导致增强类胡萝卜素形成的机制，水仙花（水仙花）花用番茄红素环化酶（LYC）抑制剂CPTA处理。在这些条件下，色素体发生了强烈的形态变化，积累的番茄红素被发现隔离在晶体中。在此过程中，总类胡萝卜素含量增加约2倍。这种增加伴随着八氢番茄红素合成酶（PSY），八氢番茄红素去饱和酶（PDS）和番茄红素环化酶（LYC）的转录水平的上调。相反，zeta-胡萝卜素去饱和酶（ZDS）不是CPTA诱导上调的目标，尽管其产物番茄红素积累。在蛋白质水平，CPTA诱导的胡萝卜素酶也被观察到，再次与ZDS的例外。番茄红素的积累，引起的CPTA处理，也伴随着β-胡萝卜素衍生物，特别是玉米黄质的减少。我们的数据表明，类胡萝卜素的生物合成可能会经历一个终产物的调节，通过一个效应可能来自β-胡萝卜素衍生物。CPTA处理后测得的脱落酸（阿坝）的浓度增加可能参与这种调节现象，指出存在一个额外的叶黄素独立的来源，这种植物激素。
To investigate mechanisms leading to enhanced carotenoid formation, daffodil (Narcissus pseudonarcissus) flowers were treated with the lycopene cyclase (LYC) inhibitor CPTA. Under these conditions, chromoplasts underwent strong morphological changes and the lycopene accumulated was found sequestered in crystals. During this process the total carotenoid content increased about 2-fold. This increase was accompanied by an upregulation of the transcript levels of phytoene synthase (PSY), phytoene desaturase (PDS) and lycopene cyclase (LYC). In contrast, zeta-carotene desaturase (ZDS) was not a target for CPTA-induced upregulation, although its product lycopene was accumulated. At the protein level, CPTA induction of carotenogenic enzymes was also observed, again with the exception of ZDS. Lycopene accumulation, caused by CPTA treatment, was also accompanied by a decrease of beta-carotene derivatives, especially zeaxanthin. Our data suggest that carotenoid biosynthesis may undergo an end-product regulation via an effector probably originating from a beta-carotene derivative. Increased concentrations of abscisic acid (ABA) measured after CPTA treatment may be involved in this regulatory phenomenon, pointing to the presence of an additional xanthophyll-independent source of this phytohormone.