STAT1 and Nmi are downstream targets of Ets-1 transcription factor in MCF-7 human breast cancer cell
STAT1 and Nmi are downstream targets of Ets-1 transcription factor in MCF-7 human breast cancer cell
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DOI：
10.1016/j.febslet.2005.06.011
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发表时间：
2005-07-18
期刊：
影响因子：
3.5
通讯作者：
Im, YH
中科院分区：
文献类型：
--
作者：
Jung, HH;Lee, J;Im, YH
Ets-1是E26病毒ets癌基因产物的细胞同源物，其作为组织特异性转录因子发挥功能。它在细胞增殖、分化、淋巴细胞发育、转化、血管生成和细胞凋亡中发挥重要作用。Ets-1通过与转录调控区中的ets结合位点结合来控制参与这些过程的关键基因的表达。在此，我们在MCF-7中瞬时过表达Ets-1，并通过cDNA微阵列分析全面搜索Ets-1的潜在下游靶点。Ets-1的过表达增强了包括STAT 1和Nmi在内的几种干扰素相关基因的表达。RT-PCR和Western blotting证实了STAT 1和Nmi mRNA和蛋白水平的增加。相反，Ets-1 siRNA降低STAT 1和Nmi蛋白的表达。在我们的瞬时转染实验中，稳定过表达Ets-1也增加了MCF-7细胞中STAT 1和Nmi的蛋白表达。综上所述，我们的结果表明，STAT 1和Nmi是MCF-7人乳腺癌细胞中Ets-1的下游靶点。(c)2005年欧洲生物化学学会联合会。All rights reserved.
Ets-1 is a cellular homologue of the product of the viral ets oncogene of the E26 virus, and it functions as a tissue-specific transcription factor. It plays an important role in cell proliferation, differentiation, lymphoid cell development, transformation, angiogenesis, and apoptosis. Ets-1 controls the expression of critical genes involved in these processes by binding to ets binding sites present in the transcriptional regulatory regions. Here, we transiently overexpressed Ets-1 in MCF-7 and comprehensively searched for potential downstream targets of Ets-1 by cDNA microarray analysis. The expressions of several interferon-related genes including STAT1 and Nmi were augmented by the overexpression of Ets-1. RT-PCR and Western blotting confirmed the increase in the levels of STAT1 and Nmi mRNA and protein. In contrast, Ets-1 siRNA decreased the expression of STAT1 and Nmi proteins. As in our transient transfection experiments, stable overexpression of Ets-1, also increased the protein expression of STAT1 and Nmi in MCF-7 cells. Taken together, our results indicate that STAT1 and Nmi are downstream targets of Ets-1 in MCF-7 human breast cancer cells. (c) 2005 Federation of European Biochemical Societies. All rights reserved.