Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus
Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus
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不同启动子对表达传染性法氏囊病病毒VP2基因的重组马立克氏病病毒1型保护功效的影响
DOI：
10.1016/j.vaccine.2016.10.008
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发表时间：
2016-11-11
期刊：
影响因子：
5.5
通讯作者：
Wang, Xiaomei
中科院分区：
文献类型：
--
作者：
Li, Kai;Liu, Yongzhen;Wang, Xiaomei
重组病毒的疫苗效力可受到许多因素的影响。因此，启动子的活性已成为影响抗原表达和保护率的主要因素之一。在本研究中，两个重组马立克氏病病毒1型（MDV 1）疫苗含有传染性法氏囊病病毒（IBDV）的VP 2基因在不同的启动子控制下，重叠fosmid DNA产生。含有在Pec启动子（CMV增强子和鸡β-肌动蛋白嵌合体启动子）控制下的VP 2基因的rMDV-Pec-VP 2病毒在鸡中表现出比使用CMV启动子的rMDV-CMV-VP 2病毒更高的VP 2表达和更强的针对IBDV的抗体应答。在无特定病原体的鸡中，经IBDV致死攻击后，rMDV-Pec-VP 2提供对发展中的死亡率、临床症状和法氏囊病变形成的完全保护，这优于rMDV-CMV-VP 2提供的保护。我们的研究结果表明，重组MDV 1疫苗对IBDV的保护效力与VP 2表达高度相关。该表达VP 2的MDV 1重组疫苗具有作为抗鸡传染性法氏囊病病毒强毒和MDV感染的双价疫苗的潜力。(C)2016爱思唯尔有限公司版权所有。
The vaccine efficacy of recombinant viruses can be influenced by many factors. Accordingly, the activity of promoters has been one of the major factors affecting the antigen expression and protection rate. In the present study, two recombinant Marek's disease virus type 1 (MDV1) vaccines containing the VP2 gene of infectious bursal disease virus (IBDV) under control of different promoters were generated from overlapping fosmid DNAs. The rMDV-Pec-VP2 virus containing the VP2 gene under control of the Pec promoter (CMV enhancer and chicken beta-actin chimera promoter) demonstrated higher VP2 expression and stronger antibody response against IBDV in chickens than the rMDV-CMV-VP2 virus using the CMV promoter. After IBDV lethal challenge in specific-pathogen-free chickens, rMDV-Pec-VP2 provided complete protection against developing mortality, clinical signs, and the formation of bursal lesions, which was better than that provided by rMDV-CMV-VP2. Our findings indicate that the protective efficacy of the recombinant MDV1 vaccine against IBDV highly correlates with VP2 expression. This recombinant MDV1 vaccine expressing VP2 could have significant potential as a bivalent vaccine against both virulent IBDV and MDV infections in chickens. (C) 2016 Elsevier Ltd. All rights reserved.