Myriad Applications of Circulating Cell-Free DNA in Precision Organ Transplant Monitoring
Myriad Applications of Circulating Cell-Free DNA in Precision Organ Transplant Monitoring
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DOI：
10.1513/annalsats.201608-634mg
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发表时间：
2017-09-01
影响因子：
8.3
通讯作者：
De Vlaminck, Iwijn
中科院分区：
文献类型：
--
作者：
Burnham, Philip;Khush, Kiran;De Vlaminck, Iwijn
实体器官移植仍然是许多终末期器官疾病的首选治疗方法，但由于急性排斥和感染而导致的并发症频繁发生，损害了其长期效益。因此，监测同种异体移植物的健康状况是移植后治疗的重要组成部分。在这里，我们回顾了循环无细胞DNA(CfDNA)在移植后排斥、感染和免疫抑制监测中的几个新应用。我们进一步讨论了cfDNA的细胞起源和显著的生物物理性质。自20世纪40年代末发现cfDNA以来，cfDNA的一个突出特性是它能够产生惊喜。我们回顾了对cfDNA表观遗传学特征的最新见解，这再次为移植监测提供了新的机会。
Solid organ transplantation remains the preferred treatment for many end-stage organ diseases, but complications due to acute rejection and infection occur frequently and undermine its long-term benefits. Monitoring of the health of the allograft is therefore a critically important component of post-transplant therapy. Here, we review several emerging applications of circulating cell-free DNA (cfDNA) in the post-transplant monitoring of rejection, infection, and immunosuppression. We further discuss the cellular origins and salient biophysical properties of cfDNA. A property of cfDNA that has been prominent since its discovery in the late 1940s is its ability to yield surprises. We review recent insights into the epigenetic features of cfDNA that yet again provide novel opportunities for transplant monitoring.