The role of regulatory B cells in Echinococcus granulosus-infected mice
The role of regulatory B cells in Echinococcus granulosus-infected mice
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调节性 B 细胞在细粒棘球绦虫感染小鼠中的作用
DOI：
10.1007/s00436-020-07025-3
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发表时间：
2021-02-01
影响因子：
2
通讯作者：
Ma，Xiumin
中科院分区：
文献类型：
--
作者：
Qi，Xinwei;Shan，Jiaoyu;Ma，Xiumin
目的探讨细粒棘球绦虫持续感染小鼠过程中调节性B细胞及其相关分子表达水平的表型变化及其与细粒棘球绦虫感染及其免疫效果的关系。实验组小鼠经腹腔注射原头节菌悬液制备细粒棘球绦虫感染小鼠模型。用流式细胞仪检测小鼠脾和外周血中调节性B细胞CD1dhiCD5+CD19hiccell和CD1dhiCD5+CD19hiIL-10+细胞的表达。用双抗体夹心法检测小鼠血清中IL-10和转化生长因子-β-1的表达。H&E染色观察小鼠肝脏病理变化，免疫组织化学方法检测小鼠肝脏中IL-10和转化生长因子-β1的表达和分布。酶联免疫吸附试验结果显示，早期感染小鼠血清IL-10和转化生长因子-β-1水平无明显变化。而在感染中晚期，小鼠血清中IL-10和转化生长因子-β-1水平显著升高(P<；0.05)。感染细粒棘球绦虫后90d，小鼠脾中CD1dhiCD5+CD19hiBreg细胞比例和CD1dhiCD5+CD19hiIL-10+Breg细胞比例均升高，提示Breg细胞在感染后期即有增殖。CD1dhiCD5+CD19调节B细胞可能是细粒棘球绦虫感染免疫抑制的原因之一。推测Bregs的抑制作用可能是通过调节细胞因子的表达，诱导抑制性细胞因子IL-10和转化生长因子β-1的分泌发挥作用。
To investigate the phenotypic changes of the expression level of regulatory B cells and related molecules during the continuous infection of Echinococcus granulosus (E. granulosus) in mice and its relationship with E. granulosus infection and its immune effect. Experimental group mice were inoculated with protoscoleces suspension via intraperitoneally injection to prepare a mouse model of E. granulosus infection. Flow cytometry was used to detect the expression of regulatory B cells CD1dhiCD5+CD19hicells and CD1dhiCD5+CD19hiIL-10+cells in spleen and peripheral blood of mice. The expressions of IL-10 and TGF-β1 in mouse serum were detected via ELISA. The liver pathological changes in mice were observed by H&E staining; Moreover, the expressions and distribution of IL-10 and TGF-β1 in mice liver were measured through immunohistochemistry. The ELISA test results showed no significant changes in serum IL-10 and TGF-β1 levels in early infected mice. However, at the middle and late stages of infection, the levels of IL-10 and TGF-β1 in the serum of mice increased significantly (P < 0.05). The proportion of CD1dhiCD5+CD19hiBreg cells and the proportion of CD1dhiCD5+CD19hiIL-10+Breg cells in the spleen of mice infected with E. granulosus were increased at 90 days after infection, which indicating that Breg cells proliferated in the late stage of infection. CD1dhiCD5+CD19hiregulatory B cells may be one of the causes of immunosuppression of E. granulosus infection. It is speculated that Bregs inhibitory effect may play a role by regulating the expression of cytokines and inducing the secretion of inhibitory cytokines IL-10 and TGF-β1.