Evaluating the selectivity of colorimetric test (Fast Blue BB salt) for the cannabinoids identification in marijuana street samples by UV-Vis, TLC, ESI(+)FT-ICR MS and ESI(+)MS/MS
Evaluating the selectivity of colorimetric test (Fast Blue BB salt) for the cannabinoids identification in marijuana street samples by UV-Vis, TLC, ESI(+)FT-ICR MS and ESI(+)MS/MS
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DOI：
10.1016/j.forc.2016.07.001
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发表时间：
2016-08-01
影响因子：
2.7
通讯作者：
Romao, Wanderson
中科院分区：
文献类型：
--
作者：
dos Santos, Nayara A.;Souza, Lindamara M.;Romao, Wanderson
大麻是世界上销售量最大的非法药物之一。源自大麻 L 的 Delta(9)-四氢大麻酚（或 Delta(9)-THC）在巴西被 2006 年 8 月 23 日起第 11.343 号法律及其异构体、盐、醚和酯所禁止。然而，Delta(9)-THC 是该植物产生致幻作用的主要原因。巴西法医警察使用的主要筛选测试是一种称为固蓝 B 或 BB 盐的比色测试，其中基本介质中出现微红色表明存在大麻素，例如 Delta(9)-THC、大麻酚 (CBN) 和/或大麻二酚 (CBD)。然而，需要更好地了解这种化学反应。本工作采用正离子模式电喷雾电离傅里叶变换离子回旋共振质谱(ESI(+)FT-ICR MS)、碰撞诱导解离(CID)实验或ESI(+)MS/MS、紫外-可见(UV-Vis)光谱和薄层色谱(TLC)对比色测试的产物进行鉴定。提出了固蓝BB盐（4-氨基-2,5-二乙氧基苯甲酰苯胺重氮化锌复盐）与Delta(9)-THC分子反应生成的产物（m/z 626）的化学结构。此外，在存在其他植物（如 Jacaranda decurrens Cham 和 Paullinia cupana Kunth）干扰的情况下评估了比色测试的选择性。 (C) 2016 Elsevier B.V. 保留所有权利。
Marijuana is one of the most marketed illicit drugs in the world. Originated from Cannabis sativa L, Delta(9)-tetrahydrocannabinol (or Delta(9)-THC) is proscribed in Brazil by the law number 11.343 from August 23rd of 2006 as well as its isomers, salts, ethers and esters. However, Delta(9)-THC is the main responsible for the hallucinogenic effects of the plant. The principal screening test used by the Brazilian Forensic Police is a colorimetric test called Fast Blue B or BB salt, in which the development of a reddish color in a basic medium indicates the presence of cannabinoids such as Delta(9)-THC, cannabinol (CBN) and/or cannabidiol (CBD). However, a better understanding of this chemical reaction is required. In this work, the products of the colorimetric test were identified by positive-ion mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(+)FT-ICR MS), collision-induced dissociation (CID) experiments or ESI(+)MS/MS, ultraviolet-visible (UV-Vis) spectroscopy and thin layer chromatography (TLC). A chemical structure was proposed for the product of the reaction (m/z 626) formed by Fast Blue BB salt (4-amino-2,5-diethoxybenzanilide diazotated zinc double salt) and Delta(9)-THC molecule. Besides, the selectivity of the colorimetric test was evaluated in presence of interferences from other plants such as Jacaranda decurrens Cham and Paullinia cupana Kunth. (C) 2016 Elsevier B.V. All rights reserved.