Cultivated corneal endothelial transplantation in a primate: Possible future clinical application in corneal endothelial regenerative medicine
Cultivated corneal endothelial transplantation in a primate: Possible future clinical application in corneal endothelial regenerative medicine
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DOI：
10.1097/ico.0b013e31817f2298
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发表时间：
2008-09-01
期刊：
影响因子：
2.8
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
--
作者：
Koizumi, Noriko;Sakamoto, Yuji;Kinoshita, Shigeru
目的：探讨一种利用灵长类动物角膜内皮细胞进行角膜内皮移植的方法。方法：采用I型胶原载体传代培养猴角膜内皮细胞。对6只猴6只眼的角膜内皮细胞进行密集刮除，4只眼植入培养的MCEC片。对照组1只眼植入不含MCECs的胶原膜，另1只眼前房注射培养的MCECs悬液。结果：MCECs形成融合的单层紧密附着的六角形细胞。表达ZO-1和Na+-K三磷酸腺苷酶。术后早期MCEC片贴附于后弹力膜，角膜透明性恢复。移植6个月后，MCEC移植的角膜仍然透明，并观察到紧密附着的六角形细胞。在1只观察时间较长的动物中，在活体镜面显微镜下观察到密度为2000个/mm(2)的多边形细胞，并保持在1600个/mm(2)的密度下。
Purpose: To review our attempt to devise a method of cultivated corneal endothelial transplantation using primates in which corneal endothelium, like that of humans, has low proliferative ability.Methods: Monkey corneal endothelial cells (MCECs) were cultivated, with Subcultures grown on collagen type I carriers. The corneal endothelia of 6 eyes of 6 monkeys were scraped intensively, after which cultivated MCEC sheets were inserted into the anterior chamber of 4 eyes. As controls, a collagen sheet without MCECs was transplanted in 1 eye of a monkey, and a suspension of cultivated MCECs was injected into the anterior chamber of 1 eye of another monkey.Results: MCECs produced a confluent monolayer of closely attached hexagonal cells. which expressed both ZO-1 and Na+-K adenosine triphosphatase. Early postoperative period MCEC sheets were attached to Descemet membrane, and corneal clarity was recovered. Six months after transplantation, MCEC-transplanted corneas remained clear, and closely attached hexagonal cells were observed. In 1 animal with longer observation, polygonal cells were observed by in vivo specular microscopy at a density of >2000 cells/mm(2) and remained > 1600 cells/mm(2) for