An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot
An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot
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DOI：
10.1107/s0021889802016709
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发表时间：
2002-12-01
影响因子：
6.1
通讯作者：
Phizackerley, RP
中科院分区：
文献类型：
--
作者：
Cohen, AE;Ellis, PJ;Phizackerley, RP
在斯坦福大学同步辐射实验室（SSRL），已经实现了一个自动化的系统，用于安装和解冻预冻晶体。它基于一个小型工业机器人和紧凑的圆柱形盒，每个盒最多可容纳96个安装在汉普顿研究样品针上的晶体。为了便于运输和存储，磁带适合在几个流行的干托运人和长期存储杜瓦瓶。一个分配杜瓦瓶在靠近光束线测角仪的液氮中最多容纳三个盒。该机器人使用永磁工具从盒中提取样本并将样本插入盒中，并使用低温钳工具将样本转移到光束线测角仪。该系统结构简单，运动部件少，工作可靠，使用方便。
An automated system for mounting and dismounting pre-frozen crystals has been implemented at the Stanford Synchrotron Radiation Laboratory (SSRL). It is based on a small industrial robot and compact cylindrical cassettes, each holding up to 96 crystals mounted on Hampton Research sample pins. For easy shipping and storage, the cassette fits inside several popular dry-shippers and long-term storage Dewars. A dispensing Dewar holds up to three cassettes in liquid nitrogen adjacent to the beamline goniometer. The robot uses a permanent magnet tool to extract samples from, and insert samples into a cassette, and a cryo-tong tool to transfer them to and from the beamline goniometer. The system is simple, with few moving parts, reliable in operation and convenient to use.