Signal regulatory protein alpha is a conserved marker for mouse and rat spermatogonial stem cells
Signal regulatory protein alpha is a conserved marker for mouse and rat spermatogonial stem cells
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DOI：
10.1093/biolre/ioad006
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发表时间：
2023-01
影响因子：
3.6
通讯作者：
Takehiro Miyazaki;Mito Kanatsu-Shinohara;M. Ema;T. Shinohara
中科院分区：
文献类型：
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作者：
Takehiro Miyazaki;Mito Kanatsu-Shinohara;M. Ema;T. Shinohara
摘要 精原干细胞（SSC）的表征因其频率低且缺乏将其与定向精原细胞区分开的特征而受到阻碍。几乎没有发现保守的 SSC 标记。为了鉴定新的 SSC 标记，我们评估了小鼠和大鼠 SSC 中的 SIRPA 表达。 SIRPA 在一小群未分化的精原细胞中表达。 SIRPA 及其配体 CD47 在培养的 SSC 中表达。补充 GDNF 和 FGF2 后 SIRPA 和 CD47 的表达上调，从而促进 SSC 自我更新。短发夹 RNA 消耗 Sirpa 会损害培养的 SSC 的增殖，并且这些细胞表现出 MAP2K1 激活和 PTPN11 磷酸化降低。免疫沉淀实验表明 SIRPA 与 PTPN11 相关。 Ptpn11 耗竭以类似于 Sirpa 耗竭的方式损害 SSC 活性。 SIRPA 在大鼠和猴睾丸的未分化精原细胞中表达。异种移植实验证明 SIRPA 在大鼠 SSC 中表达。这些结果表明，SIRPA 是一种保守的 SSC 标记，可通过 PTPN11 激活 MAP2K1 途径来促进 SSC 自我更新分裂。摘要句子 SIRPA 在小鼠和大鼠 SSC 上表达。
Abstract Characterization of spermatogonial stem cells (SSCs) has been hampered by their low frequency and lack of features that distinguish them from committed spermatogonia. Few conserved SSC markers have been discovered. To identify a new SSC marker, we evaluated SIRPA expression in mouse and rat SSCs. SIRPA was expressed in a small population of undifferentiated spermatogonia. SIRPA, and its ligand CD47 were expressed in cultured SSCs. Expression of both SIRPA and CD47 was upregulated by supplementation of GDNF and FGF2, which promoted SSC self-renewal. Sirpa depletion by short hairpin RNA impaired the proliferation of cultured SSCs, and these cells showed decreased MAP2K1 activation and PTPN11 phosphorylation. Immunoprecipitation experiments showed that SIRPA associates with PTPN11. Ptpn11 depletion impaired SSC activity in a manner similar to Sirpa depletion. SIRPA was expressed in undifferentiated spermatogonia in rat and monkey testes. Xenogenic transplantation experiments demonstrated that SIRPA is expressed in rat SSCs. These results suggest that SIRPA is a conserved SSC marker that promotes SSC self-renewal division by activating the MAP2K1 pathway via PTPN11. Summary Sentence SIRPA is expressed on mouse and rat SSCs.